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Article

Bone Marrow MSC Secretome Increases Equine Articular Chondrocyte Collagen Accumulation and Their Migratory Capacities

1
Normandie University, Unicaen, Biotargen, F-14000 Caen, France
2
Molecular Engineering and Articular Physiopathology (IMoPA), French National Center for Scientific Research (CNRS), Université de Lorraine, F-54000 Nancy, France
3
Center of Imaging and Research on Locomotor Affections on Equines (CIRALE), Unit Under Contract 957 Equine Biomechanics and Locomotor Disorders (USC 957 BPLC), French National Research Institute for Agriculture Food and Environment (INRAE), École Nationale Vétérinaire d’Alfort, F-94700 Maisons-Alfort, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to the work.
Academic Editor: Sang-Bae Han
Int. J. Mol. Sci. 2022, 23(10), 5795; https://doi.org/10.3390/ijms23105795
Received: 20 April 2022 / Revised: 17 May 2022 / Accepted: 19 May 2022 / Published: 21 May 2022
Equine osteoarthritis (OA) leads to cartilage degradation with impaired animal well-being, premature cessation of sport activity, and financial losses. Mesenchymal stem cell (MSC)-based therapies are promising for cartilage repair, but face limitations inherent to the cell itself. Soluble mediators and extracellular vesicles (EVs) secreted by MSCs are the alternatives to overcome those limitations while preserving MSC restorative properties. The effect of equine bone marrow MSC secretome on equine articular chondrocytes (eACs) was analyzed with indirect co-culture and/or MSC-conditioned media (CM). The expression of healthy cartilage/OA and proliferation markers was evaluated in eACs (monolayers or organoids). In vitro repair experiments with MSC-CM were made to evaluate the proliferation and migration of eACs. The presence of nanosized EVs in MSC-CM was appraised with nanoparticle tracking assay and transmission electron microscopy. Our results demonstrated that the MSC secretome influences eAC phenotype by increasing cartilage functionality markers and cell migration in a greater way than MSCs, which could delay OA final outcomes. This study makes acellular therapy an appealing strategy to improve equine OA treatments. However, the MSC secretome contains a wide variety of soluble mediators and small EVs, such as exosomes, and further investigation must be performed to understand the mechanisms occurring behind these promising effects. View Full-Text
Keywords: osteoarthritis; tissue engineering; acellular therapy; horse; mesenchymal stem cells; cell secretome; extracellular vesicles; in vitro repair; cartilage organoids; chondral defects osteoarthritis; tissue engineering; acellular therapy; horse; mesenchymal stem cells; cell secretome; extracellular vesicles; in vitro repair; cartilage organoids; chondral defects
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MDPI and ACS Style

Contentin, R.; Jammes, M.; Bourdon, B.; Cassé, F.; Bianchi, A.; Audigié, F.; Branly, T.; Velot, É.; Galéra, P. Bone Marrow MSC Secretome Increases Equine Articular Chondrocyte Collagen Accumulation and Their Migratory Capacities. Int. J. Mol. Sci. 2022, 23, 5795. https://doi.org/10.3390/ijms23105795

AMA Style

Contentin R, Jammes M, Bourdon B, Cassé F, Bianchi A, Audigié F, Branly T, Velot É, Galéra P. Bone Marrow MSC Secretome Increases Equine Articular Chondrocyte Collagen Accumulation and Their Migratory Capacities. International Journal of Molecular Sciences. 2022; 23(10):5795. https://doi.org/10.3390/ijms23105795

Chicago/Turabian Style

Contentin, Romain, Manon Jammes, Bastien Bourdon, Frédéric Cassé, Arnaud Bianchi, Fabrice Audigié, Thomas Branly, Émilie Velot, and Philippe Galéra. 2022. "Bone Marrow MSC Secretome Increases Equine Articular Chondrocyte Collagen Accumulation and Their Migratory Capacities" International Journal of Molecular Sciences 23, no. 10: 5795. https://doi.org/10.3390/ijms23105795

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