Towards 3D Bioprinted Spinal Cord Organoids
Abstract
:1. Introduction
2. Results
2.1. Hydrogel Gelation Time
2.2. Cell Survival and Proliferation
2.3. Cell Differentiation
2.4. Alamar Blue Evaluation
2.5. Cell and Hydrogel Imaging
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.1.1. Boundary Cap Neural Crest Stem Cell Culture
4.1.2. iPSC-Derived Motor Neuron Cultures
4.1.3. iPSC-Derived Astrocyte Cultures
4.2. Hydrogel Ink Preparation
4.3. 3D Printing of Cells
4.3.1. 3D Printing of BC
4.3.2. 3D Printing of iPSC-Derived Cells
4.4. Cell Differentiation Assay
4.5. Viability Assays
4.6. Immunohistochemistry
4.7. Live Imaging
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gelatin Percentage | ||||||
---|---|---|---|---|---|---|
mTG Concentration | 4% | 6% | 8% | 10% | 12% | 15% |
15 mg/mL | >2 h | >2 h | >2 h | 49.0 min | 32.0 min | 24.0 min |
20 mg/mL | >2 h | >2 h | 1.8 h | 47.3 min | 30.0 min | 22.3 min |
25 mg/mL | 1.7 h | 1.4 h | 1.2 h | 42.0 min | 27.0 min | 17.0 min |
30 mg/mL | 1.7 h | 1.3 h | 1 h | 38.3 min | 19.0 min | 6.3 min |
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Han, Y.; King, M.; Tikhomirov, E.; Barasa, P.; Souza, C.D.S.; Lindh, J.; Baltriukiene, D.; Ferraiuolo, L.; Azzouz, M.; Gullo, M.R.; et al. Towards 3D Bioprinted Spinal Cord Organoids. Int. J. Mol. Sci. 2022, 23, 5788. https://doi.org/10.3390/ijms23105788
Han Y, King M, Tikhomirov E, Barasa P, Souza CDS, Lindh J, Baltriukiene D, Ferraiuolo L, Azzouz M, Gullo MR, et al. Towards 3D Bioprinted Spinal Cord Organoids. International Journal of Molecular Sciences. 2022; 23(10):5788. https://doi.org/10.3390/ijms23105788
Chicago/Turabian StyleHan, Yilin, Marianne King, Evgenii Tikhomirov, Povilas Barasa, Cleide Dos Santos Souza, Jonas Lindh, Daiva Baltriukiene, Laura Ferraiuolo, Mimoun Azzouz, Maurizio R. Gullo, and et al. 2022. "Towards 3D Bioprinted Spinal Cord Organoids" International Journal of Molecular Sciences 23, no. 10: 5788. https://doi.org/10.3390/ijms23105788