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Indigo Pulverata Levis (Chung-Dae, Persicaria tinctoria) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro

Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, Korea
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Authors to whom correspondence should be addressed.
Academic Editors: Seung-Hong Lee and Ginnae Ahn
Int. J. Mol. Sci. 2022, 23(1), 553; https://doi.org/10.3390/ijms23010553
Received: 30 November 2021 / Revised: 30 December 2021 / Accepted: 1 January 2022 / Published: 5 January 2022
(This article belongs to the Special Issue Biological Interactions of Bioactive Natural Products)
Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with a type 2 T helper cell (Th2) immune response. The IndigoPulverata Levis extract (CHD) is used in traditional Southeast Asian medicine; however, its beneficial effects on AD remain uninvestigated. Therefore, we investigated the therapeutic effects of CHD in 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice and tumor necrosis factor (TNF)-α- and interferon gamma (IFN)-γ-stimulated HaCaT cells. We evaluated immune cell infiltration, skin thickness, and the serum IgE and TNF-α levels in DNCB-induced AD mice. Moreover, we measured the expression levels of pro-inflammatory cytokines, mitogen-activated protein kinase (MAPK), and the nuclear factor-kappa B (NF-κB) in the mice dorsal skin. We also studied the effect of CHD on the translocation of NF-κB p65 and inflammatory chemokines in HaCaT cells. Our in vivo results revealed that CHD reduced the dermis and epidermis thicknesses and inhibited immune cell infiltration. Furthermore, it suppressed the proinflammatory cytokine expression and MAPK and NF-κB phosphorylations in the skin tissue and decreased serum IgE and TNF-α levels. In vitro results indicated that CHD downregulated inflammatory chemokines and blocked NF-κB p65 translocation. Thus, we deduced that CHD is a potential drug candidate for AD treatment. View Full-Text
Keywords: atopic dermatitis; Indigo Pulverata Levis; immune-cell infiltration; skin thickness; NF-κB p65; proinflammatory cytokines atopic dermatitis; Indigo Pulverata Levis; immune-cell infiltration; skin thickness; NF-κB p65; proinflammatory cytokines
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MDPI and ACS Style

Min, G.-Y.; Kim, J.-H.; Kim, T.-I.; Cho, W.-K.; Yang, J.-H.; Ma, J.-Y. Indigo Pulverata Levis (Chung-Dae, Persicaria tinctoria) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro. Int. J. Mol. Sci. 2022, 23, 553. https://doi.org/10.3390/ijms23010553

AMA Style

Min G-Y, Kim J-H, Kim T-I, Cho W-K, Yang J-H, Ma J-Y. Indigo Pulverata Levis (Chung-Dae, Persicaria tinctoria) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro. International Journal of Molecular Sciences. 2022; 23(1):553. https://doi.org/10.3390/ijms23010553

Chicago/Turabian Style

Min, Ga-Yul, Ji-Hye Kim, Tae-In Kim, Won-Kyung Cho, Ju-Hye Yang, and Jin-Yeul Ma. 2022. "Indigo Pulverata Levis (Chung-Dae, Persicaria tinctoria) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro" International Journal of Molecular Sciences 23, no. 1: 553. https://doi.org/10.3390/ijms23010553

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