Colonic Mucosal Immune Activation in Mice with Ovalbumin-Induced Allergic Airway Disease: Association between Allergic Airway Disease and Irritable Bowel Syndrome
Abstract
:1. Introduction
2. Results
2.1. Allergic Airway Mouse Model Establishment
2.2. Evaluation of Cytokine Expression in Colonic Mucosa of AAD Mouse Using Quantitative RT-PCR Method (qRT-PCR)
2.3. Macroscopic Scoring of Colon Changes
2.4. Histopathological Analysis of the Colonic Mucosa
2.5. Evaluation of Intestinal Cytokine Expression Using Enzyme-Linked Immunosorbent Assay (ELISA)
2.6. Mucosal MC Counts
2.7. Tryptase Western Blotting
3. Discussion
4. Materials and Methods
4.1. AAD Mouse Model
4.2. Macroscopic Evaluation of Inflammation
4.3. qRT-PCR for Inflammatory Cytokines in the Intestinal Mucosa
4.4. Salbutamol Treatment after OVA Challenge
4.5. Histological Evaluation of Inflammation
4.6. ELISA for Inflammatory Cytokines in the Intestinal Mucosa
4.7. Evaluation of MC Count
4.8. Western Blot Analysis of Tryptase
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | Control | AAD Group | Salbutamol Group |
---|---|---|---|
Colon length, cm | 11.50 ± 0.27 | 9.171 ± 0.22 * | 11.40 ± 0.29 |
Colon weight, g | 0.491 ± 0.014 | 0.498 ± 0.015 | 0.479 ± 0.018 |
Stool score | 0.2 ± 0.133 | 1.3 ± 0.26 * | 0.5 ± 0.167 |
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Kim, S.; Keum, B.; Byun, J.; Kim, B.; Lee, K.; Yeon, J.; Lee, J.; Choi, H.; Kim, E.; Jeen, Y.; et al. Colonic Mucosal Immune Activation in Mice with Ovalbumin-Induced Allergic Airway Disease: Association between Allergic Airway Disease and Irritable Bowel Syndrome. Int. J. Mol. Sci. 2022, 23, 181. https://doi.org/10.3390/ijms23010181
Kim S, Keum B, Byun J, Kim B, Lee K, Yeon J, Lee J, Choi H, Kim E, Jeen Y, et al. Colonic Mucosal Immune Activation in Mice with Ovalbumin-Induced Allergic Airway Disease: Association between Allergic Airway Disease and Irritable Bowel Syndrome. International Journal of Molecular Sciences. 2022; 23(1):181. https://doi.org/10.3390/ijms23010181
Chicago/Turabian StyleKim, Sanghyun, Bora Keum, Junhyoung Byun, Byoungjae Kim, Kijeong Lee, Jiwoo Yeon, Jaemin Lee, Hyuksoon Choi, Eunsun Kim, Yoontae Jeen, and et al. 2022. "Colonic Mucosal Immune Activation in Mice with Ovalbumin-Induced Allergic Airway Disease: Association between Allergic Airway Disease and Irritable Bowel Syndrome" International Journal of Molecular Sciences 23, no. 1: 181. https://doi.org/10.3390/ijms23010181