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Review

Proteomics: A Tool to Study Platelet Function

1
Leibniz-Institut für Analytische Wissenschaften—ISAS—e.V, Bunsen-Kirchhoff-Straße 11, 44139 Dortmund, Germany
2
Department of Immunodynamics, Institute of Experimental Immunology and Imaging, University Hospital Essen, Hufelandstrasse 55, 45147 Essen, Germany
3
Department of Biotechnology, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, Croatia
4
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Torez pr. 44, 194223 St. Petersburg, Russia
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West German Heart and Vascular Center, Department of Cardiology and Vascular Medicine, University Hospital Essen, Hufelandstrasse 55, 45147 Essen, Germany
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III. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany
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Institut für Immunologie und Transfusionsmedizin, Universitätsmedizin Greifswald, Sauerbruchstraße, 17475 Greifswald, Germany
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Institute of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany
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Medizinisches Proteom-Center (MPC), Medizinische Fakultät, Ruhr-Universität Bochum, 44801 Bochum, Germany
10
Department of Chemistry, College of Physical Sciences, University of Aberdeen, Aberdeen AB24 3FX, UK
*
Authors to whom correspondence should be addressed.
Academic Editors: Valeria Gasperi, Isabella Savini and Maria Valeria Catani
Int. J. Mol. Sci. 2021, 22(9), 4776; https://doi.org/10.3390/ijms22094776
Received: 16 February 2021 / Revised: 22 April 2021 / Accepted: 22 April 2021 / Published: 30 April 2021
(This article belongs to the Special Issue Molecular Research on Platelet Activity in Health and Disease 3.0)
Platelets are components of the blood that are highly reactive, and they quickly respond to multiple physiological and pathophysiological processes. In the last decade, it became clear that platelets are the key components of circulation, linking hemostasis, innate, and acquired immunity. Protein composition, localization, and activity are crucial for platelet function and regulation. The current state of mass spectrometry-based proteomics has tremendous potential to identify and quantify thousands of proteins from a minimal amount of material, unravel multiple post-translational modifications, and monitor platelet activity during drug treatments. This review focuses on the role of proteomics in understanding the molecular basics of the classical and newly emerging functions of platelets. including the recently described role of platelets in immunology and the development of COVID-19.The state-of-the-art proteomic technologies and their application in studying platelet biogenesis, signaling, and storage are described, and the potential of newly appeared trapped ion mobility spectrometry (TIMS) is highlighted. Additionally, implementing proteomic methods in platelet transfusion medicine, and as a diagnostic and prognostic tool, is discussed. View Full-Text
Keywords: platelets; signaling; mass spectrometry; LC-MS/MS; PTMs; phosphoproteomics; targeted proteomics; platelet transfusion proteomics; precision medicine platelets; signaling; mass spectrometry; LC-MS/MS; PTMs; phosphoproteomics; targeted proteomics; platelet transfusion proteomics; precision medicine
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MDPI and ACS Style

Shevchuk, O.; Begonja, A.J.; Gambaryan, S.; Totzeck, M.; Rassaf, T.; Huber, T.B.; Greinacher, A.; Renne, T.; Sickmann, A. Proteomics: A Tool to Study Platelet Function. Int. J. Mol. Sci. 2021, 22, 4776. https://doi.org/10.3390/ijms22094776

AMA Style

Shevchuk O, Begonja AJ, Gambaryan S, Totzeck M, Rassaf T, Huber TB, Greinacher A, Renne T, Sickmann A. Proteomics: A Tool to Study Platelet Function. International Journal of Molecular Sciences. 2021; 22(9):4776. https://doi.org/10.3390/ijms22094776

Chicago/Turabian Style

Shevchuk, Olga, Antonija Jurak Begonja, Stepan Gambaryan, Matthias Totzeck, Tienush Rassaf, Tobias B. Huber, Andreas Greinacher, Thomas Renne, and Albert Sickmann. 2021. "Proteomics: A Tool to Study Platelet Function" International Journal of Molecular Sciences 22, no. 9: 4776. https://doi.org/10.3390/ijms22094776

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