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Article

Mesenchymal Stromal Cell Therapy in Novel Porcine Model of Diffuse Liver Damage Induced by Repeated Biliary Obstruction

1
Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 76, 323 00 Pilsen, Czech Republic
2
Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 00 Pilsen, Czech Republic
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Department of Surgery, Medical School and Teaching Hospital in Pilsen, Charles University, Alej Svobody 80, 304 60 Pilsen, Czech Republic
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Department of Anesthesiology and Intensive Care, Medical School and Teaching Hospital in Pilsen, Charles University, Alej Svobody 80, 304 60 Pilsen, Czech Republic
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Department of Clinical Biochemistry and Hematology, Medical School and Teaching Hospital in Pilsen, Charles University, Alej Svobody 80, 304 60 Pilsen, Czech Republic
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Department of Radiology, Medical School and Teaching Hospital in Pilsen, Charles University, Alej Svobody 80, 304 60 Pilsen, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Marco Tatullo
Int. J. Mol. Sci. 2021, 22(9), 4304; https://doi.org/10.3390/ijms22094304
Received: 31 March 2021 / Revised: 18 April 2021 / Accepted: 19 April 2021 / Published: 21 April 2021
In liver surgery, biliary obstruction can lead to secondary biliary cirrhosis, a life-threatening disease with liver transplantation as the only curative treatment option. Mesenchymal stromal cells (MSC) have been shown to improve liver function in both acute and chronic liver disease models. This study evaluated the effect of allogenic MSC transplantation in a large animal model of repeated biliary obstruction followed by partial hepatectomy. MSC transplantation supported the growth of regenerated liver tissue after 14 days (MSC group, n = 10: from 1087 ± 108 (0 h) to 1243 ± 92 mL (14 days); control group, n = 11: from 1080 ± 95 (0 h) to 1100 ± 105 mL (14 days), p = 0.016), with a lower volume fraction of hepatocytes in regenerated liver tissue compared to resected liver tissue (59.5 ± 10.2% vs. 70.2 ± 5.6%, p < 0.05). Volume fraction of connective tissue, blood vessels and bile vessels in regenerated liver tissue, serum levels of liver enzymes (AST, ALT, ALP and GGT) and liver metabolites (albumin, bilirubin, urea and creatinine), as well as plasma levels of IL-6, IL-8, TNF-α and TGF-β, were not affected by MSC transplantation. In our novel, large animal (pig) model of repeated biliary obstruction followed by partial hepatectomy, MSC transplantation promoted growth of liver tissue without any effect on liver function. This study underscores the importance of translating results between small and large animal models as well as the careful translation of results from animal model into human medicine. View Full-Text
Keywords: secondary biliary cirrhosis; hepatectomy; pig model; mesenchymal stromal cell; quantitative histology secondary biliary cirrhosis; hepatectomy; pig model; mesenchymal stromal cell; quantitative histology
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MDPI and ACS Style

Vištejnová, L.; Liška, V.; Kumar, A.; Křečková, J.; Vyčítal, O.; Brůha, J.; Beneš, J.; Kolinko, Y.; Blassová, T.; Tonar, Z.; Brychtová, M.; Karlíková, M.; Racek, J.; Mírka, H.; Hošek, P.; Lysák, D.; Králíčková, M. Mesenchymal Stromal Cell Therapy in Novel Porcine Model of Diffuse Liver Damage Induced by Repeated Biliary Obstruction. Int. J. Mol. Sci. 2021, 22, 4304. https://doi.org/10.3390/ijms22094304

AMA Style

Vištejnová L, Liška V, Kumar A, Křečková J, Vyčítal O, Brůha J, Beneš J, Kolinko Y, Blassová T, Tonar Z, Brychtová M, Karlíková M, Racek J, Mírka H, Hošek P, Lysák D, Králíčková M. Mesenchymal Stromal Cell Therapy in Novel Porcine Model of Diffuse Liver Damage Induced by Repeated Biliary Obstruction. International Journal of Molecular Sciences. 2021; 22(9):4304. https://doi.org/10.3390/ijms22094304

Chicago/Turabian Style

Vištejnová, Lucie; Liška, Václav; Kumar, Arvind; Křečková, Jana; Vyčítal, Ondřej; Brůha, Jan; Beneš, Jan; Kolinko, Yaroslav; Blassová, Tereza; Tonar, Zbyněk; Brychtová, Michaela; Karlíková, Marie; Racek, Jaroslav; Mírka, Hynek; Hošek, Petr; Lysák, Daniel; Králíčková, Milena. 2021. "Mesenchymal Stromal Cell Therapy in Novel Porcine Model of Diffuse Liver Damage Induced by Repeated Biliary Obstruction" Int. J. Mol. Sci. 22, no. 9: 4304. https://doi.org/10.3390/ijms22094304

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