Next Article in Journal
Image- and Fluorescence-Based Test Shows Oxidant-Dependent Damages in Red Blood Cells and Enables Screening of Potential Protective Molecules
Next Article in Special Issue
Prolonged Chronic Consumption of a High Fat with Sucrose Diet Alters the Morphology of the Small Intestine
Previous Article in Journal
Out-of-Field Hippocampus from Partial-Body Irradiated Mice Displays Changes in Multi-Omics Profile and Defects in Neurogenesis
Previous Article in Special Issue
Modeling Intestinal Epithelial Response to Interferon-γ in Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 8
10.3390/ijms22084292
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Crohn’s Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat

by
Ana Madeira
1,2,†,
Carolina Serena
1,2,†,
Miriam Ejarque
1,2,
Elsa Maymó-Masip
1,2,
Monica Millan
1,2,3,
M. Carmen Navarro-Ruiz
4,5,6,
Rocío Guzmán-Ruiz
4,5,6,
María M. Malagón
4,5,6,
Eloy Espin
7,
Marc Martí
7,
Margarita Menacho
8,
Ana Megía
1,2,
Joan Vendrell
1,2,9,* and
Sonia Fernández-Veledo
1,2,*
1
Department of Research, Institut d’Investigació Sanitària Pere Virgili, Hospital Universitari de Tarragona Joan XXIII, 43005 Tarragona, Spain
2
Instituto de Salud Carlos III, CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), 08029 Madrid, Spain
3
Department of Surgery, Colorectal Surgery Unit, Hospital Universitari i Politècnic La Fe, 46026 Valencia, Spain
4
Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), University of Cordoba, Reina Sofia University Hospital, Edificio IMIBIC, Avda. Menéndez Pidal s/n, 14004 Cordoba, Spain
5
Department of Cell Biology, Physiology and Immunology, University of Cordoba, 14004 Cordoba, Spain
6
CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain
7
Colorectal Surgery Unit, General Surgery Service, Hospital Vall d’Hebron, Universitat Autònoma de Barcelona, 08035 Barcelona, Spain
8
Digestive Unit, Hospital Universitari Joan XXIII, 43007 Tarragona, Spain
9
Department Medicine and Surgery, Medicine School, Universitat Rovira i Virgili, 43003 Tarragona, Spain
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2021, 22(8), 4292; https://doi.org/10.3390/ijms22084292
Submission received: 10 March 2021 / Revised: 13 April 2021 / Accepted: 16 April 2021 / Published: 20 April 2021
(This article belongs to the Special Issue Role of Environmental and Metabolic Factors in Inflammatory Bowel Disorders)
Browse Figures
Review Reports Versions Notes

Abstract

Our understanding of the interplay between human adipose tissue and the immune system is limited. The mesothelium, an immunologically active structure, emerged as a source of visceral adipose tissue. After investigating the mesothelial properties of human visceral and subcutaneous adipose tissue and their progenitors, we explored whether the dysfunctional obese and Crohn’s disease environments influence the mesothelial/mesenchymal properties of their adipocyte precursors, as well as their ability to mount an immune response. Using a tandem transcriptomic/proteomic approach, we evaluated the mesothelial and mesenchymal expression profiles in adipose tissue, both in subjects covering a wide range of body-mass indexes and in Crohn’s disease patients. We also isolated adipose tissue precursors (adipose-derived stem cells, ASCs) to assess their mesothelial/mesenchymal properties, as well as their antigen-presenting features. Human visceral tissue presented a mesothelial phenotype not detected in the subcutaneous fat. Only ASCs from mesenteric adipose tissue, named creeping fat, had a significantly higher expression of the hallmark mesothelial genes mesothelin (MSLN) and Wilms’ tumor suppressor gene 1 (WT1), supporting a mesothelial nature of these cells. Both lean and Crohn’s disease visceral ASCs expressed equivalent surface percentages of the antigen-presenting molecules human leucocyte antigen—DR isotype (HLA-DR) and CD86. However, lean-derived ASCs were predominantly HLA-DR dim, whereas in Crohn’s disease, the HLA-DR bright subpopulation was increased 3.2-fold. Importantly, the mesothelial-enriched Crohn’s disease precursors activated CD4+ T-lymphocytes. Our study evidences a mesothelial signature in the creeping fat of Crohn’s disease patients and its progenitor cells, the latter being able to present antigens and orchestrate an immune response.
Keywords: Crohn’s disease; adipose tissue; mesothelium; adipose-derived stem cells Crohn’s disease; adipose tissue; mesothelium; adipose-derived stem cells

Share and Cite

MDPI and ACS Style

Madeira, A.; Serena, C.; Ejarque, M.; Maymó-Masip, E.; Millan, M.; Navarro-Ruiz, M.C.; Guzmán-Ruiz, R.; Malagón, M.M.; Espin, E.; Martí, M.; et al. Crohn’s Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat. Int. J. Mol. Sci. 2021, 22, 4292. https://doi.org/10.3390/ijms22084292

AMA Style

Madeira A, Serena C, Ejarque M, Maymó-Masip E, Millan M, Navarro-Ruiz MC, Guzmán-Ruiz R, Malagón MM, Espin E, Martí M, et al. Crohn’s Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat. International Journal of Molecular Sciences. 2021; 22(8):4292. https://doi.org/10.3390/ijms22084292

Chicago/Turabian Style

Madeira, Ana, Carolina Serena, Miriam Ejarque, Elsa Maymó-Masip, Monica Millan, M. Carmen Navarro-Ruiz, Rocío Guzmán-Ruiz, María M. Malagón, Eloy Espin, Marc Martí, and et al. 2021. "Crohn’s Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat" International Journal of Molecular Sciences 22, no. 8: 4292. https://doi.org/10.3390/ijms22084292

APA Style

Madeira, A., Serena, C., Ejarque, M., Maymó-Masip, E., Millan, M., Navarro-Ruiz, M. C., Guzmán-Ruiz, R., Malagón, M. M., Espin, E., Martí, M., Menacho, M., Megía, A., Vendrell, J., & Fernández-Veledo, S. (2021). Crohn’s Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat. International Journal of Molecular Sciences, 22(8), 4292. https://doi.org/10.3390/ijms22084292

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop