SH3BP2 Deficiency Ameliorates Murine Systemic Lupus Erythematosus
Abstract
:1. Introduction
2. Results
2.1. SH3BP2 Deficiency Improves Splenomegaly and Glomerular Proliferative Changes in Lupus-Prone Mice
2.2. SH3BP2 Deficiency Suppresses Autoantibody Production
2.3. SH3BP2 Deficiency Blunts the Aberrant Accumulation of CD3+B220+CD4-CD8- Double-Negative T (DNT) Cells
2.4. B Cell-Specific SH3BP2 Deficiency Does Not Improve the Clinical and Immunological Phenotypes
2.5. SH3BP2 Deficiency Does Not Directly Affect the Activation of CD4+ T Cells
2.6. SH3BP2 Deficiency Modulates Optimal In Vitro Differentiation of Bone Marrow-Derived Dendritic Cells (DCs)
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Animal Study: Analysis of the Lupus-Prone Mice
4.3. Western Blot Analysis
4.4. Isolation of Mouse Primary T Cells and B Cells
4.5. Urinary Protein Assessment
4.6. Histopathologic Assessment of Kidney
4.7. Enzyme-Linked Immunosorbent Assay (ELISA) for Serum Anti-dsDNA Antibody and IgM-RF
4.8. Serum Immunoglobulin Measurement
4.9. Flow Cytometry for Immune Cell Subsets
4.10. Functional Analyses of CD4+ T Cells
4.11. Generation of Dendritic Cells (DCs) from Bone Marrow Cells
4.12. Flow Cytometric Analyses of DCs
4.13. Culture of Bone Marrow-Derived Macrophages
4.14. Real-Time Quantitative Polymerase Chain Reaction (qPCR)
4.15. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
References
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Kawahara, K.; Mukai, T.; Iseki, M.; Nagasu, A.; Nagasu, H.; Akagi, T.; Tsuji, S.; Hiramatsu-Asano, S.; Ueki, Y.; Ishihara, K.; et al. SH3BP2 Deficiency Ameliorates Murine Systemic Lupus Erythematosus. Int. J. Mol. Sci. 2021, 22, 4169. https://doi.org/10.3390/ijms22084169
Kawahara K, Mukai T, Iseki M, Nagasu A, Nagasu H, Akagi T, Tsuji S, Hiramatsu-Asano S, Ueki Y, Ishihara K, et al. SH3BP2 Deficiency Ameliorates Murine Systemic Lupus Erythematosus. International Journal of Molecular Sciences. 2021; 22(8):4169. https://doi.org/10.3390/ijms22084169
Chicago/Turabian StyleKawahara, Kyoko, Tomoyuki Mukai, Masanori Iseki, Akiko Nagasu, Hajime Nagasu, Takahiko Akagi, Shoko Tsuji, Sumie Hiramatsu-Asano, Yasuyoshi Ueki, Katsuhiko Ishihara, and et al. 2021. "SH3BP2 Deficiency Ameliorates Murine Systemic Lupus Erythematosus" International Journal of Molecular Sciences 22, no. 8: 4169. https://doi.org/10.3390/ijms22084169