The PI-3-Kinase P110α Catalytic Subunit of T Lymphocytes Modulates Collagen-Induced Arthritis
Abstract
:1. Introduction
2. Results
2.1. P110α−/−ΔT Mice Show a Lower Prevalence of CIA
2.2. Impact of T-Cell-Specific Loss of p110α in Collagen-Specific Abs and Serum IL-6
2.3. Ex Vivo Response of Lymph Node Cells in CIA in the p110α−/−ΔT Mouse Model
2.4. Analysis of Lymph Node Subpopulations in p110α−/−ΔT Mice under CIA
2.5. Analysis of the Immune Response to Collagen II in p110α−/−ΔT Mice before the Arthritis Onset
2.6. Effect of Genetic Deletion of p110α in Homing Markers Expression and ICOS-Dependent T-Cell Signaling
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Collagen-Induced Arthritis: Induction and Assessment
4.3. Analysis of T-Cell Responses
4.4. Measurement of Anti-Collagen Antibodies
4.5. Analysis of Cytokine Production
4.6. Analysis of Surface and Intracellular Markers by Flow Cytometry
4.7. CD4+ T-Cell Blasts, Generation, Activation and Western Blot
4.8. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Montes-Casado, M.; Ojeda, G.; Criado, G.; Rojo, J.M.; Portolés, P. The PI-3-Kinase P110α Catalytic Subunit of T Lymphocytes Modulates Collagen-Induced Arthritis. Int. J. Mol. Sci. 2021, 22, 6405. https://doi.org/10.3390/ijms22126405
Montes-Casado M, Ojeda G, Criado G, Rojo JM, Portolés P. The PI-3-Kinase P110α Catalytic Subunit of T Lymphocytes Modulates Collagen-Induced Arthritis. International Journal of Molecular Sciences. 2021; 22(12):6405. https://doi.org/10.3390/ijms22126405
Chicago/Turabian StyleMontes-Casado, María, Gloria Ojeda, Gabriel Criado, José M. Rojo, and Pilar Portolés. 2021. "The PI-3-Kinase P110α Catalytic Subunit of T Lymphocytes Modulates Collagen-Induced Arthritis" International Journal of Molecular Sciences 22, no. 12: 6405. https://doi.org/10.3390/ijms22126405