Next Article in Journal
Dimethyl Trisulfide Diminishes Traumatic Neuropathic Pain Acting on TRPA1 Receptors in Mice
Previous Article in Journal
Therapeutic Potential for Regulation of the Nuclear Factor Kappa-B Transcription Factor p65 to Prevent Cellular Senescence and Activation of Pro-Inflammatory in Mesenchymal Stem Cells
Previous Article in Special Issue
Adipocyte-Derived Extracellular Vesicles: State of the Art
Review

Beyond the Extracellular Vesicles: Technical Hurdles, Achieved Goals and Current Challenges When Working on Adipose Cells

Institute for Tumor Immunology, Center for Tumor Biology and Immunology (ZTI), Philipps University of Marburg, 35043 Marburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: María Pardo Pérez
Int. J. Mol. Sci. 2021, 22(7), 3362; https://doi.org/10.3390/ijms22073362
Received: 26 February 2021 / Revised: 18 March 2021 / Accepted: 22 March 2021 / Published: 25 March 2021
(This article belongs to the Special Issue Role of the Extracellular Vesicles in Obesity and Related Diseases)
Adipose tissue and its crosstalk with other organs plays an essential role in the metabolic homeostasis of the entire body. Alteration of this communication (i.e., due to obesity) is related to the development of several comorbidities including type 2 diabetes, cardiovascular diseases, or cancer. Within the adipose depot, adipocytes are the main cell type and thus the main source of secreted molecules, which exert modulating effects not only at a local but also at a systemic level. Extracellular vesicles (EVs) have recently emerged as important mediators in cell–cell communication and account for part of the cellular secretome. In recent years, there has been a growing body of research on adipocyte-derived extracellular vesicles (Ad-EVs). However, there is still a lack of standardized methodological approaches, especially regarding primary adipocytes. In this review, we will provide an outline of crucial aspects when working on adipose-derived material, with a special focus on primary adipocytes. In parallel, we will point out current methodological challenges in the EV field and how they impact the transcriptomic, proteomic and functional evaluations of Ad-EVs. View Full-Text
Keywords: adipocytes; differential centrifugation; extracellular vesicles; isolation methods; primary culture; single-vesicle analysis adipocytes; differential centrifugation; extracellular vesicles; isolation methods; primary culture; single-vesicle analysis
Show Figures

Figure 1

MDPI and ACS Style

Gómez-Serrano, M.; Ponath, V.; Preußer, C.; Pogge von Strandmann, E. Beyond the Extracellular Vesicles: Technical Hurdles, Achieved Goals and Current Challenges When Working on Adipose Cells. Int. J. Mol. Sci. 2021, 22, 3362. https://doi.org/10.3390/ijms22073362

AMA Style

Gómez-Serrano M, Ponath V, Preußer C, Pogge von Strandmann E. Beyond the Extracellular Vesicles: Technical Hurdles, Achieved Goals and Current Challenges When Working on Adipose Cells. International Journal of Molecular Sciences. 2021; 22(7):3362. https://doi.org/10.3390/ijms22073362

Chicago/Turabian Style

Gómez-Serrano, María, Viviane Ponath, Christian Preußer, and Elke Pogge von Strandmann. 2021. "Beyond the Extracellular Vesicles: Technical Hurdles, Achieved Goals and Current Challenges When Working on Adipose Cells" International Journal of Molecular Sciences 22, no. 7: 3362. https://doi.org/10.3390/ijms22073362

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop