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Article

Neonatal Mesenchymal Stem Cell Treatment Improves Myelination Impaired by Global Perinatal Asphyxia in Rats

1
Molecular & Clinical Pharmacology Program, ICBM, Faculty of Medicine, University of Chile, Santiago 8380453, Chile
2
Faculty of Medicine, School of Pharmacy, Universidad Andres Bello, Santiago 8370149, Chile
3
Centro de Medicina Regenerativa, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Av. Las Condes 12438, Lo Barnechea, Santiago 7710162, Chile
4
Department of Neuroscience, Faculty of Medicine, University of Chile, Santiago 8380453, Chile
*
Authors to whom correspondence should be addressed.
Academic Editor: Elena Rybnikova
Int. J. Mol. Sci. 2021, 22(6), 3275; https://doi.org/10.3390/ijms22063275
Received: 8 February 2021 / Revised: 7 March 2021 / Accepted: 15 March 2021 / Published: 23 March 2021
(This article belongs to the Special Issue Brain Hypoxia: Mechanisms of Resilience and Tolerance)
The effect of perinatal asphyxia (PA) on oligodendrocyte (OL), neuroinflammation, and cell viability was evaluated in telencephalon of rats at postnatal day (P)1, 7, and 14, a period characterized by a spur of neuronal networking, evaluating the effect of mesenchymal stem cell (MSCs)-treatment. The issue was investigated with a rat model of global PA, mimicking a clinical risk occurring under labor. PA was induced by immersing fetus-containing uterine horns into a water bath for 21 min (AS), using sibling-caesarean-delivered fetuses (CS) as controls. Two hours after delivery, AS and CS neonates were injected with either 5 μL of vehicle (10% plasma) or 5 × 104 MSCs into the lateral ventricle. Samples were assayed for myelin-basic protein (MBP) levels; Olig-1/Olig-2 transcriptional factors; Gglial phenotype; neuroinflammation, and delayed cell death. The main effects were observed at P7, including: (i) A decrease of MBP-immunoreactivity in external capsule, corpus callosum, cingulum, but not in fimbriae of hippocampus; (ii) an increase of Olig-1-mRNA levels; (iii) an increase of IL-6-mRNA, but not in protein levels; (iv) an increase in cell death, including OLs; and (v) MSCs treatment prevented the effect of PA on myelination, OLs number, and cell death. The present findings show that PA induces regional- and developmental-dependent changes on myelination and OLs maturation. Neonatal MSCs treatment improves survival of mature OLs and myelination in telencephalic white matter. View Full-Text
Keywords: hypomyelination; oligodendrocyte; myelination; neonatal asphyxia/ischemia; apoptosis; mesenchymal stem cells; telencephalon; rat brain; neuroinflammation; periventricular leukomalacia hypomyelination; oligodendrocyte; myelination; neonatal asphyxia/ischemia; apoptosis; mesenchymal stem cells; telencephalon; rat brain; neuroinflammation; periventricular leukomalacia
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MDPI and ACS Style

Tapia-Bustos, A.; Lespay-Rebolledo, C.; Vío, V.; Pérez-Lobos, R.; Casanova-Ortiz, E.; Ezquer, F.; Herrera-Marschitz, M.; Morales, P. Neonatal Mesenchymal Stem Cell Treatment Improves Myelination Impaired by Global Perinatal Asphyxia in Rats. Int. J. Mol. Sci. 2021, 22, 3275. https://doi.org/10.3390/ijms22063275

AMA Style

Tapia-Bustos A, Lespay-Rebolledo C, Vío V, Pérez-Lobos R, Casanova-Ortiz E, Ezquer F, Herrera-Marschitz M, Morales P. Neonatal Mesenchymal Stem Cell Treatment Improves Myelination Impaired by Global Perinatal Asphyxia in Rats. International Journal of Molecular Sciences. 2021; 22(6):3275. https://doi.org/10.3390/ijms22063275

Chicago/Turabian Style

Tapia-Bustos, Andrea; Lespay-Rebolledo, Carolyne; Vío, Valentina; Pérez-Lobos, Ronald; Casanova-Ortiz, Emmanuel; Ezquer, Fernando; Herrera-Marschitz, Mario; Morales, Paola. 2021. "Neonatal Mesenchymal Stem Cell Treatment Improves Myelination Impaired by Global Perinatal Asphyxia in Rats" Int. J. Mol. Sci. 22, no. 6: 3275. https://doi.org/10.3390/ijms22063275

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