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Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of A. thaliana

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Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, 80-307 Gdansk, Poland
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Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
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Department of Cell Biology, Department of Cellular and Molecular Biology, Nicolaus Copernicus University, 87-100 Torun, Poland
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Department of Plant Breeding, Swedish University of Agricultural Sciences, 230-53 Alnarp, Sweden
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Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, 750-07 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Mamoru Sugita
Int. J. Mol. Sci. 2021, 22(6), 3006; https://doi.org/10.3390/ijms22063006
Received: 4 February 2021 / Revised: 10 March 2021 / Accepted: 12 March 2021 / Published: 16 March 2021
(This article belongs to the Section Biochemistry)
Arabidopsis thaliana possesses two acyl-CoA:lysophosphatidylethanolamine acyltransferases, LPEAT1 and LPEAT2, which are encoded by At1g80950 and At2g45670 genes, respectively. Both single lpeat2 mutant and double lpeat1 lpeat2 mutant plants exhibit a variety of conspicuous phenotypes, including dwarfed growth. Confocal microscopic analysis of tobacco suspension-cultured cells transiently transformed with green fluorescent protein-tagged versions of LPEAT1 or LPEAT2 revealed that LPEAT1 is localized to the endoplasmic reticulum (ER), whereas LPEAT2 is localized to both Golgi and late endosomes. Considering that the primary product of the reaction catalyzed by LPEATs is phosphatidylethanolamine, which is known to be covalently conjugated with autophagy-related protein ATG8 during a key step of the formation of autophagosomes, we investigated the requirements for LPEATs to engage in autophagic activity in Arabidopsis. Knocking out of either or both LPEAT genes led to enhanced accumulation of the autophagic adaptor protein NBR1 and decreased levels of both ATG8a mRNA and total ATG8 protein. Moreover, we detected significantly fewer membrane objects in the vacuoles of lpeat1 lpeat2 double mutant mesophyll cells than in vacuoles of control plants. However, contrary to what has been reported on autophagy deficient plants, the lpeat mutants displayed a prolonged life span compared to wild type, including delayed senescence. View Full-Text
Keywords: autophagy; NBR1; ATG8; acyl-CoA:lysophosphatidylethanolamine acyltransferases; Arabidopsis thaliana; senescence autophagy; NBR1; ATG8; acyl-CoA:lysophosphatidylethanolamine acyltransferases; Arabidopsis thaliana; senescence
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MDPI and ACS Style

Jasieniecka-Gazarkiewicz, K.; Demski, K.; Gidda, S.K.; Klińska, S.; Niedojadło, J.; Lager, I.; Carlsson, A.S.; Minina, E.A.; Mullen, R.T.; Bozhkov, P.V.; Stymne, S.; Banaś, A. Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of A. thaliana. Int. J. Mol. Sci. 2021, 22, 3006. https://doi.org/10.3390/ijms22063006

AMA Style

Jasieniecka-Gazarkiewicz K, Demski K, Gidda SK, Klińska S, Niedojadło J, Lager I, Carlsson AS, Minina EA, Mullen RT, Bozhkov PV, Stymne S, Banaś A. Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of A. thaliana. International Journal of Molecular Sciences. 2021; 22(6):3006. https://doi.org/10.3390/ijms22063006

Chicago/Turabian Style

Jasieniecka-Gazarkiewicz, Katarzyna, Kamil Demski, Satinder K. Gidda, Sylwia Klińska, Janusz Niedojadło, Ida Lager, Anders S. Carlsson, Elena A. Minina, Robert T. Mullen, Peter V. Bozhkov, Sten Stymne, and Antoni Banaś. 2021. "Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of A. thaliana" International Journal of Molecular Sciences 22, no. 6: 3006. https://doi.org/10.3390/ijms22063006

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