Loss of Nckx3 Exacerbates Experimental DSS-Induced Colitis in Mice through p53/NF-κB Pathway
Abstract
:1. Introduction
2. Results
2.1. Nckx3 KO Mice Exhibit Distinct Transcriptional Profiles of Proliferation- and Inflammation-Associated Genes
2.2. Nckx3 Loss Impairs the Morphology and Cell Proliferation of the Gut
2.3. Nckx3 Loss Promotes Acute DSS-Induced Colitis
2.4. Nckx3 Deficiency Increased the Production of Dextran Sodium Sulfate (DSS)-Induced Proinflammatory Mediators
2.5. Nckx3 Loss Upregulated NF-κB Signaling in Acute DSS-Induced Colitis
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Microarray Assay
4.3. DSS-Induced Colitis
4.4. Colitis Scores and Histologic Analysis
4.5. Immunofluorescence
4.6. RNA Extraction and Quantitative Real-Time PCR
4.7. Western Blot Analysis
4.8. ELISA Assay
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Tran, D.N.; Go, S.M.; Park, S.-M.; Jung, E.-M.; Jeung, E.-B. Loss of Nckx3 Exacerbates Experimental DSS-Induced Colitis in Mice through p53/NF-κB Pathway. Int. J. Mol. Sci. 2021, 22, 2645. https://doi.org/10.3390/ijms22052645
Tran DN, Go SM, Park S-M, Jung E-M, Jeung E-B. Loss of Nckx3 Exacerbates Experimental DSS-Induced Colitis in Mice through p53/NF-κB Pathway. International Journal of Molecular Sciences. 2021; 22(5):2645. https://doi.org/10.3390/ijms22052645
Chicago/Turabian StyleTran, Dinh Nam, Seon Myeong Go, Seon-Mi Park, Eui-Man Jung, and Eui-Bae Jeung. 2021. "Loss of Nckx3 Exacerbates Experimental DSS-Induced Colitis in Mice through p53/NF-κB Pathway" International Journal of Molecular Sciences 22, no. 5: 2645. https://doi.org/10.3390/ijms22052645