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The Role of Estradiol in Traumatic Brain Injury: Mechanism and Treatment Potential
Article

Potential Neuroprotective Mechanisms of Methamphetamine Treatment in Traumatic Brain Injury Defined by Large-Scale IonStar-Based Quantitative Proteomics

by 1,2, 1,2, 2,3, 2,4, 5,* and 1,2,*
1
Department of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, USA
2
New York State Center of Excellence in Bioinformatics and Life Sciences, Buffalo, NY 14203, USA
3
Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Institute, Buffalo, NY 14203, USA
4
Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14203, USA
5
Department of Neurosurgery, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14203, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Antonio Pisani
Int. J. Mol. Sci. 2021, 22(5), 2246; https://doi.org/10.3390/ijms22052246
Received: 26 January 2021 / Revised: 18 February 2021 / Accepted: 20 February 2021 / Published: 24 February 2021
Although traumatic brain injury (TBI) causes hospitalizations and mortality worldwide, there are no approved neuroprotective treatments, partly due to a poor understanding of the molecular mechanisms underlying TBI neuropathology and neuroprotection. We previously reported that the administration of low-dose methamphetamine (MA) induced significant functional/cognitive improvements following severe TBI in rats. We further demonstrated that MA mediates neuroprotection in part, via dopamine-dependent activation of the PI3K-AKT pathway. Here, we further investigated the proteomic changes within the rat cortex and hippocampus following mild TBI (TM), severe TBI (TS), or severe TBI plus MA treatment (TSm) compared to sham operated controls. We identified 402 and 801 altered proteins (APs) with high confidence in cortical and hippocampal tissues, respectively. The overall profile of APs observed in TSm rats more closely resembled those seen in TM rather than TS rats. Pathway analysis suggested beneficial roles for acute signaling through IL-6, TGFβ, and IL-1β. Moreover, changes in fibrinogen levels observed in TSm rats suggested a potential role for these proteins in reducing/preventing TBI-induced coagulopathies. These data facilitate further investigations to identify specific pathways and proteins that may serve as key targets for the development of neuroprotective therapies. View Full-Text
Keywords: traumatic brain injury; methamphetamine; large-scale proteomics; mass spectrometry; IonStar traumatic brain injury; methamphetamine; large-scale proteomics; mass spectrometry; IonStar
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MDPI and ACS Style

Shen, S.; Zhang, M.; Ma, M.; Rasam, S.; Poulsen, D.; Qu, J. Potential Neuroprotective Mechanisms of Methamphetamine Treatment in Traumatic Brain Injury Defined by Large-Scale IonStar-Based Quantitative Proteomics. Int. J. Mol. Sci. 2021, 22, 2246. https://doi.org/10.3390/ijms22052246

AMA Style

Shen S, Zhang M, Ma M, Rasam S, Poulsen D, Qu J. Potential Neuroprotective Mechanisms of Methamphetamine Treatment in Traumatic Brain Injury Defined by Large-Scale IonStar-Based Quantitative Proteomics. International Journal of Molecular Sciences. 2021; 22(5):2246. https://doi.org/10.3390/ijms22052246

Chicago/Turabian Style

Shen, Shichen, Ming Zhang, Min Ma, Sailee Rasam, David Poulsen, and Jun Qu. 2021. "Potential Neuroprotective Mechanisms of Methamphetamine Treatment in Traumatic Brain Injury Defined by Large-Scale IonStar-Based Quantitative Proteomics" International Journal of Molecular Sciences 22, no. 5: 2246. https://doi.org/10.3390/ijms22052246

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