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Article

Accumulation of the Auxin Precursor Indole-3-Acetamide Curtails Growth through the Repression of Ribosome-Biogenesis and Development-Related Transcriptional Networks

1
Institut für Biologie, Bereich Pflanzenwissenschaften, Karl-Franzens Universität Graz, 8010 Graz, Austria
2
Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria Y Alimentación (INIA), Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain
3
Umeå Plant Science Center, Umeå University, 90736 Umeå, Sweden
4
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, 44780 Bochum, Germany
5
Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM), 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Int. J. Mol. Sci. 2021, 22(4), 2040; https://doi.org/10.3390/ijms22042040
Received: 20 January 2021 / Revised: 16 February 2021 / Accepted: 17 February 2021 / Published: 18 February 2021
The major auxin, indole-3-acetic acid (IAA), is associated with a plethora of growth and developmental processes including embryo development, expansion growth, cambial activity, and the induction of lateral root growth. Accumulation of the auxin precursor indole-3-acetamide (IAM) induces stress related processes by stimulating abscisic acid (ABA) biosynthesis. How IAM signaling is controlled is, at present, unclear. Here, we characterize the ami1rooty double mutant, that we initially generated to study the metabolic and phenotypic consequences of a simultaneous genetic blockade of the indole glucosinolate and IAM pathways in Arabidopsisthaliana. Our mass spectrometric analyses of the mutant revealed that the combination of the two mutations is not sufficient to fully prevent the conversion of IAM to IAA. The detected strong accumulation of IAM was, however, recognized to substantially impair seed development. We further show by genome-wide expression studies that the double mutant is broadly affected in its translational capacity, and that a small number of plant growth regulating transcriptional circuits are repressed by the high IAM content in the seed. In accordance with the previously described growth reduction in response to elevated IAM levels, our data support the hypothesis that IAM is a growth repressing counterpart to IAA. View Full-Text
Keywords: Arabidopsis thaliana; indole-3-acetic acid; indole-3-acetamide; indole glucosinolate; seed maturation; seed size; ribosome biogenesis; plant growth Arabidopsis thaliana; indole-3-acetic acid; indole-3-acetamide; indole glucosinolate; seed maturation; seed size; ribosome biogenesis; plant growth
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MDPI and ACS Style

Sánchez-Parra, B.; Pérez-Alonso, M.-M.; Ortiz-García, P.; Moya-Cuevas, J.; Hentrich, M.; Pollmann, S. Accumulation of the Auxin Precursor Indole-3-Acetamide Curtails Growth through the Repression of Ribosome-Biogenesis and Development-Related Transcriptional Networks. Int. J. Mol. Sci. 2021, 22, 2040. https://doi.org/10.3390/ijms22042040

AMA Style

Sánchez-Parra B, Pérez-Alonso M-M, Ortiz-García P, Moya-Cuevas J, Hentrich M, Pollmann S. Accumulation of the Auxin Precursor Indole-3-Acetamide Curtails Growth through the Repression of Ribosome-Biogenesis and Development-Related Transcriptional Networks. International Journal of Molecular Sciences. 2021; 22(4):2040. https://doi.org/10.3390/ijms22042040

Chicago/Turabian Style

Sánchez-Parra, Beatriz, Marta-Marina Pérez-Alonso, Paloma Ortiz-García, José Moya-Cuevas, Mathias Hentrich, and Stephan Pollmann. 2021. "Accumulation of the Auxin Precursor Indole-3-Acetamide Curtails Growth through the Repression of Ribosome-Biogenesis and Development-Related Transcriptional Networks" International Journal of Molecular Sciences 22, no. 4: 2040. https://doi.org/10.3390/ijms22042040

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