Blue Light Induces Impaired Autophagy through Nucleotide-Binding Oligomerization Domain 2 Activation on the Mouse Ocular Surface
Abstract
:1. Introduction
2. Results
2.1. ROS Levels on the Ocular Surface
2.2. MDA Levels on the Ocular Surface
2.3. NOD2 Activation in Corneal and Conjunctival Tissues
2.4. Western Blot for Autophagy in Corneal and Conjunctival Tissues
2.5. Counts of Apoptotic Cells in Corneal Tissue
2.6. Corneal Epithelial Damage
3. Discussion
4. Materials and Methods
4.1. Designs of Mouse Model and Experiments
4.2. Measurement of ROS Production in the Cornea and Conjunctiva
4.3. ELISA
4.4. Immunofluorescent Staining
4.5. Western Blot
4.6. Histology
4.7. TUNEL Staining
4.8. Ocular Surface Parameters
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cornea (pmol/mL) | Conjunctiva (pmol/mL) | |
---|---|---|
WT | 7.6 ± 1.3 | 8.2 ± 2.3 |
NOD2-KO | 8.0 ± 1.6 | 7.1 ± 1.1 |
WT+BL | 15.0 ± 2.4 *,† | 14.0 ± 2.5 *,† |
NOD2-KO+BL | 14.2 ± 3.0 *,† | 11.8 ± 2.2 *,† |
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Li, Y.; Jin, R.; Li, L.; Choi, J.S.; Kim, J.; Yoon, H.J.; Park, J.H.; Yoon, K.C. Blue Light Induces Impaired Autophagy through Nucleotide-Binding Oligomerization Domain 2 Activation on the Mouse Ocular Surface. Int. J. Mol. Sci. 2021, 22, 2015. https://doi.org/10.3390/ijms22042015
Li Y, Jin R, Li L, Choi JS, Kim J, Yoon HJ, Park JH, Yoon KC. Blue Light Induces Impaired Autophagy through Nucleotide-Binding Oligomerization Domain 2 Activation on the Mouse Ocular Surface. International Journal of Molecular Sciences. 2021; 22(4):2015. https://doi.org/10.3390/ijms22042015
Chicago/Turabian StyleLi, Ying, Rujun Jin, Lan Li, Ji Suk Choi, Jonghwa Kim, Hyeon Jeong Yoon, Jong Hwan Park, and Kyung Chul Yoon. 2021. "Blue Light Induces Impaired Autophagy through Nucleotide-Binding Oligomerization Domain 2 Activation on the Mouse Ocular Surface" International Journal of Molecular Sciences 22, no. 4: 2015. https://doi.org/10.3390/ijms22042015
APA StyleLi, Y., Jin, R., Li, L., Choi, J. S., Kim, J., Yoon, H. J., Park, J. H., & Yoon, K. C. (2021). Blue Light Induces Impaired Autophagy through Nucleotide-Binding Oligomerization Domain 2 Activation on the Mouse Ocular Surface. International Journal of Molecular Sciences, 22(4), 2015. https://doi.org/10.3390/ijms22042015