Next Article in Journal
Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation
Previous Article in Journal
Pax3 and Pax7 Exhibit Distinct and Overlapping Functions in Marking Muscle Satellite Cells and Muscle Repair in a Marine Teleost, Sebastes schlegelii
Previous Article in Special Issue
Blue Light Induces Impaired Autophagy through Nucleotide-Binding Oligomerization Domain 2 Activation on the Mouse Ocular Surface
 
 
Article

Characterization of Type I Interferon-Associated Chemokines and Cytokines in Lacrimal Glands of Nonobese Diabetic Mice

1
Stead Family Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
2
Immunology Graduate Program, University of Iowa, Iowa City, IA 52242, USA
3
Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
4
Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
5
Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
6
Max McGee National Research Center for Juvenile Diabetes, Medical College of Wisconsin, Milwaukee, WI 53226, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Cintia S. De Paiva and Stephen C. Pflugfelder
Int. J. Mol. Sci. 2021, 22(7), 3767; https://doi.org/10.3390/ijms22073767
Received: 9 March 2021 / Accepted: 30 March 2021 / Published: 5 April 2021
(This article belongs to the Special Issue Mechanisms of Dry Eye Diseases)
Type I interferons (IFNs) are required for spontaneous lacrimal gland inflammation in the nonobese diabetic (NOD) mouse model of Sjögren’s disease, but the consequences of type I IFN signaling are not well-defined. Here, we use RNA sequencing to define cytokine and chemokine genes upregulated in lacrimal glands of NOD mice in a type I IFN-dependent manner. Interleukin (IL)-21 was the highest differentially expressed cytokine gene, and Il21 knockout NOD mice were relatively protected from lacrimal gland inflammation. We defined a set of chemokines upregulated early in disease including Cxcl9 and Cxcl10, which share a receptor, CXCR3. CXCR3+ T cells were enriched in lacrimal glands with a dominant proportion of CXCR3+ regulatory T cells. Together these data define the early cytokine and chemokine signals associated with type I IFN-signaling in the development of lacrimal gland inflammation in NOD mice providing insight into the role of type I IFN in autoimmunity development. View Full-Text
Keywords: Sjögren’s disease; lacrimal gland; nonobese diabetic mice; type I interferon; interleukin-21; chemokines Sjögren’s disease; lacrimal gland; nonobese diabetic mice; type I interferon; interleukin-21; chemokines
Show Figures

Figure 1

MDPI and ACS Style

Allred, M.-G.; Chimenti, M.S.; Ciecko, A.E.; Chen, Y.-G.; Lieberman, S.M. Characterization of Type I Interferon-Associated Chemokines and Cytokines in Lacrimal Glands of Nonobese Diabetic Mice. Int. J. Mol. Sci. 2021, 22, 3767. https://doi.org/10.3390/ijms22073767

AMA Style

Allred M-G, Chimenti MS, Ciecko AE, Chen Y-G, Lieberman SM. Characterization of Type I Interferon-Associated Chemokines and Cytokines in Lacrimal Glands of Nonobese Diabetic Mice. International Journal of Molecular Sciences. 2021; 22(7):3767. https://doi.org/10.3390/ijms22073767

Chicago/Turabian Style

Allred, Merri-Grace, Michael S. Chimenti, Ashley E. Ciecko, Yi-Guang Chen, and Scott M. Lieberman. 2021. "Characterization of Type I Interferon-Associated Chemokines and Cytokines in Lacrimal Glands of Nonobese Diabetic Mice" International Journal of Molecular Sciences 22, no. 7: 3767. https://doi.org/10.3390/ijms22073767

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop