Prolyl Oligopeptidase Regulates Dopamine Transporter Oligomerization and Phosphorylation in a PKC- and ERK-Independent Manner
Abstract
:1. Introduction
2. Results
2.1. The Effect of PREP and α-Synuclein on Phosphorylated and Oligomeric DAT, and Phosphorylation of ERK
2.2. The Effect of PREP Inhibitor KYP-2047 on DAT and Phosphorylation of ERK
2.3. The Effect of PREP Inhibitor KYP-2047 on PKC Activity
2.4. PREP and DAT as Regulators of Dopamine Uptake
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Lines
4.3. Plasmids
4.4. Western Blotting
4.5. PKC Activity Assay
4.6. 3H-Dopamine Uptake Assay
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Julku, U.H.; Jäntti, M.; Svarcbahs, R.; Myöhänen, T.T. Prolyl Oligopeptidase Regulates Dopamine Transporter Oligomerization and Phosphorylation in a PKC- and ERK-Independent Manner. Int. J. Mol. Sci. 2021, 22, 1777. https://doi.org/10.3390/ijms22041777
Julku UH, Jäntti M, Svarcbahs R, Myöhänen TT. Prolyl Oligopeptidase Regulates Dopamine Transporter Oligomerization and Phosphorylation in a PKC- and ERK-Independent Manner. International Journal of Molecular Sciences. 2021; 22(4):1777. https://doi.org/10.3390/ijms22041777
Chicago/Turabian StyleJulku, Ulrika H., Maria Jäntti, Reinis Svarcbahs, and Timo T. Myöhänen. 2021. "Prolyl Oligopeptidase Regulates Dopamine Transporter Oligomerization and Phosphorylation in a PKC- and ERK-Independent Manner" International Journal of Molecular Sciences 22, no. 4: 1777. https://doi.org/10.3390/ijms22041777