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Article

A Proteomics Analysis of Calmodulin-Binding Proteins in Dictyostelium discoideum during the Transition from Unicellular Growth to Multicellular Development

by 1,†, 1,† and 2,*,†
1
Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON K9L 0G2, Canada
2
Department of Biology, Trent University, Peterborough, ON K9L 0G2, Canada
*
Author to whom correspondence should be addressed.
All authors contributed equally to this paper.
Academic Editor: William Cho
Int. J. Mol. Sci. 2021, 22(4), 1722; https://doi.org/10.3390/ijms22041722
Received: 16 December 2020 / Revised: 22 January 2021 / Accepted: 5 February 2021 / Published: 9 February 2021
(This article belongs to the Special Issue Calmodulin Binding Proteins)
Calmodulin (CaM) is an essential calcium-binding protein within eukaryotes. CaM binds to calmodulin-binding proteins (CaMBPs) and influences a variety of cellular and developmental processes. In this study, we used immunoprecipitation coupled with mass spectrometry (LC-MS/MS) to reveal over 500 putative CaM interactors in the model organism Dictyostelium discoideum. Our analysis revealed several known CaMBPs in Dictyostelium and mammalian cells (e.g., myosin, calcineurin), as well as many novel interactors (e.g., cathepsin D). Gene ontology (GO) term enrichment and Search Tool for the Retrieval of Interacting proteins (STRING) analyses linked the CaM interactors to several cellular and developmental processes in Dictyostelium including cytokinesis, gene expression, endocytosis, and metabolism. The primary localizations of the CaM interactors include the nucleus, ribosomes, vesicles, mitochondria, cytoskeleton, and extracellular space. These findings are not only consistent with previous work on CaM and CaMBPs in Dictyostelium, but they also provide new insight on their diverse cellular and developmental roles in this model organism. In total, this study provides the first in vivo catalogue of putative CaM interactors in Dictyostelium and sheds additional light on the essential roles of CaM and CaMBPs in eukaryotes. View Full-Text
Keywords: calmodulin; calmodulin-binding proteins; Dictyostelium discoideum; immunoprecipitation; mass spectrometry calmodulin; calmodulin-binding proteins; Dictyostelium discoideum; immunoprecipitation; mass spectrometry
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MDPI and ACS Style

Kim, W.D.; Yap, S.Q.; Huber, R.J. A Proteomics Analysis of Calmodulin-Binding Proteins in Dictyostelium discoideum during the Transition from Unicellular Growth to Multicellular Development. Int. J. Mol. Sci. 2021, 22, 1722. https://doi.org/10.3390/ijms22041722

AMA Style

Kim WD, Yap SQ, Huber RJ. A Proteomics Analysis of Calmodulin-Binding Proteins in Dictyostelium discoideum during the Transition from Unicellular Growth to Multicellular Development. International Journal of Molecular Sciences. 2021; 22(4):1722. https://doi.org/10.3390/ijms22041722

Chicago/Turabian Style

Kim, William D., Shyong Q. Yap, and Robert J. Huber. 2021. "A Proteomics Analysis of Calmodulin-Binding Proteins in Dictyostelium discoideum during the Transition from Unicellular Growth to Multicellular Development" International Journal of Molecular Sciences 22, no. 4: 1722. https://doi.org/10.3390/ijms22041722

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