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Article

Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy

1
Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
2
Department of Physics, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan
3
Department of Biotechnology, College of Life Sciences, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga 525-8577, Japan
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Department of Biomolecular Chemistry, Kyoto Prefectural University, Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan
5
Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan
*
Authors to whom correspondence should be addressed.
Academic Editor: Zongchao Jia
Int. J. Mol. Sci. 2021, 22(4), 1697; https://doi.org/10.3390/ijms22041697
Received: 23 January 2021 / Accepted: 4 February 2021 / Published: 8 February 2021
FtsZ is a key protein in bacterial cell division and is assembled into filamentous architectures. FtsZ filaments are thought to regulate bacterial cell division and have been investigated using many types of imaging techniques such as atomic force microscopy (AFM), but the time scale of the method was too long to trace the filament formation process. Development of high-speed AFM enables us to achieve sub-second time resolution and visualize the formation and dissociation process of FtsZ filaments. The analysis of the growth and dissociation rates of the C-terminal truncated FtsZ (FtsZt) filaments indicate the net growth and dissociation of FtsZt filaments in the growth and dissociation conditions, respectively. We also analyzed the curvatures of the full-length FtsZ (FtsZf) and FtsZt filaments, and the comparative analysis indicated the straight-shape preference of the FtsZt filaments than those of FtsZf. These findings provide insights into the fundamental dynamic behavior of FtsZ protofilaments and bacterial cell division. View Full-Text
Keywords: bacterial cell division; Staphylococcus aureus; FtsZ; high-speed atomic force microscopy bacterial cell division; Staphylococcus aureus; FtsZ; high-speed atomic force microscopy
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MDPI and ACS Style

Fujita, J.; Sugiyama, S.; Terakado, H.; Miyazaki, M.; Ozawa, M.; Ueda, N.; Kuroda, N.; Tanaka, S.-i.; Yoshizawa, T.; Uchihashi, T.; Matsumura, H. Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy. Int. J. Mol. Sci. 2021, 22, 1697. https://doi.org/10.3390/ijms22041697

AMA Style

Fujita J, Sugiyama S, Terakado H, Miyazaki M, Ozawa M, Ueda N, Kuroda N, Tanaka S-i, Yoshizawa T, Uchihashi T, Matsumura H. Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy. International Journal of Molecular Sciences. 2021; 22(4):1697. https://doi.org/10.3390/ijms22041697

Chicago/Turabian Style

Fujita, Junso, Shogo Sugiyama, Haruna Terakado, Maho Miyazaki, Mayuki Ozawa, Nanami Ueda, Natsuko Kuroda, Shun-ichi Tanaka, Takuya Yoshizawa, Takayuki Uchihashi, and Hiroyoshi Matsumura. 2021. "Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy" International Journal of Molecular Sciences 22, no. 4: 1697. https://doi.org/10.3390/ijms22041697

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