Next Article in Journal
Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
Next Article in Special Issue
Emerging Role of T-cell Receptor Constant β Chain-1 (TRBC1) Expression in the Flow Cytometric Diagnosis of T-cell Malignancies
Previous Article in Journal
Tumor Metabolic Reprogramming by Adipokines as a Critical Driver of Obesity-Associated Cancer Progression
Previous Article in Special Issue
Centrifugation Removes a Population of Large Vesicles, or “Macroparticles,” Intermediate in Size to RBCs and Microvesicles
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 3
10.3390/ijms22031445
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA

by
Irina Zamulaeva
1,*,
Elena Selivanova
1,
Olga Matchuk
1,
Valentina Kiseleva
1,
Liana Mkrtchyan
2 and
Ludmila Krikunova
2
1
Department of Radiation Biochemistry, A. Tsyb Medical Radiological Research Center—Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Korolev str.-4, 249036 Obninsk, Russia
2
Department of Radiation and Combined Treatment of Gynecological Diseases, A. Tsyb Medical Radiological Research Center, Korolev str.-4, 249036 Obninsk, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(3), 1445; https://doi.org/10.3390/ijms22031445
Submission received: 10 December 2020 / Revised: 24 January 2021 / Accepted: 28 January 2021 / Published: 1 February 2021
(This article belongs to the Special Issue Flow Cytometry and Its Applications to Molecular Biology and Diagnosis)
Browse Figures
Versions Notes

Abstract

Radio- and chemoresistance of cancer stem cells (CSCs) is considered as one of the possible causes of adverse results of chemoradiotherapy for various malignancies, including cervical cancer. However, little is known about quantitative changes in the CSC subpopulation in the course of treatment and mechanisms for individual response of CSCs to therapy. The purpose of the study was to evaluate the association of radiation response of cervical CSCs with clinical and morphological parameters of disease and features of human papillomavirus (HPV) infection. The proportion of CD44+CD24low CSCs was determined by flow cytometry in cervical scrapings from 55 patients with squamous cell carcinoma of uterine cervix before treatment and after fractionated irradiation at a total dose of 10 Gy. Real-time PCR assay was used to evaluate molecular parameters of HPV DNA. Post-radiation increase in the CSC proportion was found in 47.3% of patients. Clinical and morphological parameters (stage, status of lymph node involvement, and histological type) were not significantly correlated with radiation changes in the CSC proportion. Single- and multifactor analyses revealed two independent indicators affecting the radiation response of CSCs: initial proportion of CSCs and physical status of HPV DNA (R = 0.86, p = 0.001 for the multiple regression model in the whole).
Keywords: cervical cancer; radiotherapy; cancer stem cells; human papillomavirus; flow cytometry cervical cancer; radiotherapy; cancer stem cells; human papillomavirus; flow cytometry

Share and Cite

MDPI and ACS Style

Zamulaeva, I.; Selivanova, E.; Matchuk, O.; Kiseleva, V.; Mkrtchyan, L.; Krikunova, L. Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA. Int. J. Mol. Sci. 2021, 22, 1445. https://doi.org/10.3390/ijms22031445

AMA Style

Zamulaeva I, Selivanova E, Matchuk O, Kiseleva V, Mkrtchyan L, Krikunova L. Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA. International Journal of Molecular Sciences. 2021; 22(3):1445. https://doi.org/10.3390/ijms22031445

Chicago/Turabian Style

Zamulaeva, Irina, Elena Selivanova, Olga Matchuk, Valentina Kiseleva, Liana Mkrtchyan, and Ludmila Krikunova. 2021. "Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA" International Journal of Molecular Sciences 22, no. 3: 1445. https://doi.org/10.3390/ijms22031445

APA Style

Zamulaeva, I., Selivanova, E., Matchuk, O., Kiseleva, V., Mkrtchyan, L., & Krikunova, L. (2021). Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA. International Journal of Molecular Sciences, 22(3), 1445. https://doi.org/10.3390/ijms22031445

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop