Next Article in Journal
Machine Learning Analysis of Longevity-Associated Gene Expression Landscapes in Mammals
Next Article in Special Issue
Mast Cell and Astrocyte Hemichannels and Their Role in Alzheimer’s Disease, ALS, and Harmful Stress Conditions
Previous Article in Journal
A Novel Digestive α-Amylase from Blue Crab (Portunus segnis) Viscera: Purification, Biochemical Characterization and Application for the Improvement of Antioxidant Potential of Oat Flour
Previous Article in Special Issue
Cardiac Connexin-43 Hemichannels and Pannexin1 Channels: Provocative Antiarrhythmic Targets
Article

Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling

1
Centro Interdisciplinario de Neurociencia de Valparaíso, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso 2360102, Chile
2
Programa de Doctorado en Ciencias, Mención Neurociencia, Universidad de Valparaíso, Valparaíso 2340000, Chile
3
Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Valparaíso 2360102, Chile
4
Centro de Investigación Farmacopea Chilena, Valparaíso 2360102, Chile
5
Laboratorio de Biología de la Reproducción y del Desarrollo, Instituto de Biología, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso 2340000, Chile
6
Laboratorio de Fisiología Molecular y Biofísica, Facultad de Odontología, Universidad de Valparaíso, Valparaíso 2360004, Chile
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(3), 1069; https://doi.org/10.3390/ijms22031069
Received: 30 November 2020 / Revised: 13 January 2021 / Accepted: 14 January 2021 / Published: 22 January 2021
Wound healing is a dynamic process required to maintain skin integrity and which relies on the precise migration of different cell types. A key molecule that regulates this process is ATP. However, the mechanisms involved in extracellular ATP management are poorly understood, particularly in the human dermis. Here, we explore the role, in human fibroblast migration during wound healing, of Pannexin 1 channels and their relationship with purinergic signals and in vivo cell surface filamentous actin dynamics. Using siRNA against Panx isoforms and different Panx1 channel inhibitors, we demonstrate in cultured human dermal fibroblasts that the absence or inhibition of Panx1 channels accelerates cell migration, increases single-cell motility, and promotes actin redistribution. These changes occur through a mechanism that involves the release of ATP to the extracellular space through a Panx1-dependent mechanism and the activation of the purinergic receptor P2X7. Together, these findings point to a pivotal role of Panx1 channels in skin fibroblast migration and suggest that these channels could be a useful pharmacological target to promote damaged skin healing. View Full-Text
Keywords: pannexin 1; actin cytoskeleton; cell migration; wound healing; human dermal fibroblasts; purinergic receptor pannexin 1; actin cytoskeleton; cell migration; wound healing; human dermal fibroblasts; purinergic receptor
Show Figures

Figure 1

MDPI and ACS Style

Flores-Muñoz, C.; Maripillán, J.; Vásquez-Navarrete, J.; Novoa-Molina, J.; Ceriani, R.; Sánchez, H.A.; Abbott, A.C.; Weinstein-Oppenheimer, C.; Brown, D.I.; Cárdenas, A.M.; García, I.E.; Martínez, A.D. Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling. Int. J. Mol. Sci. 2021, 22, 1069. https://doi.org/10.3390/ijms22031069

AMA Style

Flores-Muñoz C, Maripillán J, Vásquez-Navarrete J, Novoa-Molina J, Ceriani R, Sánchez HA, Abbott AC, Weinstein-Oppenheimer C, Brown DI, Cárdenas AM, García IE, Martínez AD. Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling. International Journal of Molecular Sciences. 2021; 22(3):1069. https://doi.org/10.3390/ijms22031069

Chicago/Turabian Style

Flores-Muñoz, Carolina; Maripillán, Jaime; Vásquez-Navarrete, Jacqueline; Novoa-Molina, Joel; Ceriani, Ricardo; Sánchez, Helmuth A.; Abbott, Ana C.; Weinstein-Oppenheimer, Caroline; Brown, Donald I.; Cárdenas, Ana M.; García, Isaac E.; Martínez, Agustín D. 2021. "Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling" Int. J. Mol. Sci. 22, no. 3: 1069. https://doi.org/10.3390/ijms22031069

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop