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Antimicrobial Resistance and Inorganic Nanoparticles
Article

Hyaluronic Acid Derivative Molecular Weight-Dependent Synthesis and Antimicrobial Effect of Hybrid Silver Nanoparticles

Grup de Biotecnologia Molecular i Industrial, Department of Chemical Engineering, Universitat Politècnica de Catalunya, Rambla Sant Nebridi 22, 08222 Terrassa, Spain
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Author to whom correspondence should be addressed.
Academic Editors: Monica Terracciano, Ilaria Rea, Nicola Borbone and Chiara Tramontano
Int. J. Mol. Sci. 2021, 22(24), 13428; https://doi.org/10.3390/ijms222413428
Received: 23 November 2021 / Revised: 8 December 2021 / Accepted: 9 December 2021 / Published: 14 December 2021
(This article belongs to the Special Issue Functional Nanomaterials for Healthcare)
Silver nanoparticles (Ag NPs) appeared as promising antimicrobial candidates to face the development of antibiotic resistance. Although reported as toxic towards mammalian cells, their combination with biomolecules have shown reduced toxicity, while maintaining the antimicrobial function. Herein, hyaluronic acid (HA) with low (40 kDa), medium (200 and 600 kDa) and high (2 MDa) molecular weight (Mw) was modified with adipic acid dihydrazide (ADH) and used as reducing and capping agents to synthesise antimicrobial hybrid Ag NPs. The Mw of the polymer played a crucial role in the morphology, size and antibacterial activity of the Ag NPs. The 600 and 200 kDa HA-ADH-Ag NPs were able to reduce the Escherichia coli and Staphylococcus aureus concentration by more than 3 logs, while the 40 kDa NPs reached ~2 logs reduction. The 2 MDa HA-ADH failed to form homogenous NPs with strong bactericidal activity. A mechanistic study of the interaction with a model bacterial membrane using Langmuir isotherms confirmed the greater interaction between bacteria and higher Mw polymers and the effect of the NP’s morphology. The nanocomposites low toxicity to human skin cells was demonstrated in vitro, showing more than 90% cell viability after incubation with the NPs. View Full-Text
Keywords: hyaluronic acid; adipic acid dihydrazide; silver nanoparticles; polymer molecular weight; antimicrobial hyaluronic acid; adipic acid dihydrazide; silver nanoparticles; polymer molecular weight; antimicrobial
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MDPI and ACS Style

Ferreres, G.; Pérez-Rafael, S.; Torrent-Burgués, J.; Tzanov, T. Hyaluronic Acid Derivative Molecular Weight-Dependent Synthesis and Antimicrobial Effect of Hybrid Silver Nanoparticles. Int. J. Mol. Sci. 2021, 22, 13428. https://doi.org/10.3390/ijms222413428

AMA Style

Ferreres G, Pérez-Rafael S, Torrent-Burgués J, Tzanov T. Hyaluronic Acid Derivative Molecular Weight-Dependent Synthesis and Antimicrobial Effect of Hybrid Silver Nanoparticles. International Journal of Molecular Sciences. 2021; 22(24):13428. https://doi.org/10.3390/ijms222413428

Chicago/Turabian Style

Ferreres, Guillem, Sílvia Pérez-Rafael, Juan Torrent-Burgués, and Tzanko Tzanov. 2021. "Hyaluronic Acid Derivative Molecular Weight-Dependent Synthesis and Antimicrobial Effect of Hybrid Silver Nanoparticles" International Journal of Molecular Sciences 22, no. 24: 13428. https://doi.org/10.3390/ijms222413428

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