GM1 Is Cytoprotective in GPR37-Expressing Cells and Downregulates Signaling
Abstract
:1. Introduction
2. Results
2.1. N2aGPR37-eGFP Cells Expressed Decreased Levels of GM1
2.2. N2aGPR37-eGFP Cells Were Selectively Protected by GM1 Treatment
2.3. GM1 Decreased GPR37 Signaling
2.4. GPR37 Expression Level Was Not Changed with GM1 Treatment
3. Discussion
4. Materials and Methods
4.1. Cellculture
4.2. Quantification Total Levels of GM1
4.3. Quantification of GM1 Expression in Plasma Membrane
4.4. Resazurin Assay
4.5. qPCR
4.6. Signaling
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hertz, E.; Saarinen, M.; Svenningsson, P. GM1 Is Cytoprotective in GPR37-Expressing Cells and Downregulates Signaling. Int. J. Mol. Sci. 2021, 22, 12859. https://doi.org/10.3390/ijms222312859
Hertz E, Saarinen M, Svenningsson P. GM1 Is Cytoprotective in GPR37-Expressing Cells and Downregulates Signaling. International Journal of Molecular Sciences. 2021; 22(23):12859. https://doi.org/10.3390/ijms222312859
Chicago/Turabian StyleHertz, Ellen, Marcus Saarinen, and Per Svenningsson. 2021. "GM1 Is Cytoprotective in GPR37-Expressing Cells and Downregulates Signaling" International Journal of Molecular Sciences 22, no. 23: 12859. https://doi.org/10.3390/ijms222312859