Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup

Wang, Jing; Xu, Yujuan; Qin, Chunjun; Hu, Jing; Yin, Jian; Guo, Xi

doi:10.3390/ijms222312746

Open AccessArticle

Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup

by

Jing Wang

^1,†,

Yujuan Xu

^2,†,

Chunjun Qin

²,

Jing Hu

^3,*,

Jian Yin

² and

Xi Guo

^1,*

¹

TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457, China

²

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Ave. 1800, Wuxi 214122, China

³

Wuxi School of Medicine, Jiangnan University, Lihu Ave. 1800, Wuxi 214122, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2021, 22(23), 12746; https://doi.org/10.3390/ijms222312746

Submission received: 22 September 2021 / Revised: 24 November 2021 / Accepted: 24 November 2021 / Published: 25 November 2021

(This article belongs to the Special Issue Complex Carbohydrates and Glycoconjugates: Structure, Functions and Applications 2021)

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Abstract

The O-antigen is the outermost component of the lipopolysaccharide layer in Gram-negative bacteria, and the variation of O-antigen structure provides the basis for bacterial serological diversity. Here, we determined the O-antigen structure of an Escherichia coli strain, LL004, which is totally different from all of the E. coli serogroups. The tetrasaccharide repeating unit was determined as →4)-β-d-Galp-(1→3)-β-d-GlcpNAc6OAc(~70%)-(1→3)-β-d-GalpA-(1→3)-β-d-GalpNAc-(1→ with monosaccharide analysis and NMR spectra. We also characterized the O-antigen gene cluster of LL004, and sequence analysis showed that it correlated well with the O-antigen structure. Deletion and complementation testing further confirmed its role in O-antigen biosynthesis, and indicated that the O-antigen of LL004 is assembled via the Wzx/Wzy dependent pathway. Our findings, in combination, suggest that LL004 should represent a novel serogroup of E. coli.

Keywords: Escherichia coli; serogroup; O-antigen; structure; O-antigen gene cluster

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MDPI and ACS Style

Wang, J.; Xu, Y.; Qin, C.; Hu, J.; Yin, J.; Guo, X. Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup. Int. J. Mol. Sci. 2021, 22, 12746. https://doi.org/10.3390/ijms222312746

AMA Style

Wang J, Xu Y, Qin C, Hu J, Yin J, Guo X. Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup. International Journal of Molecular Sciences. 2021; 22(23):12746. https://doi.org/10.3390/ijms222312746

Chicago/Turabian Style

Wang, Jing, Yujuan Xu, Chunjun Qin, Jing Hu, Jian Yin, and Xi Guo. 2021. "Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup" International Journal of Molecular Sciences 22, no. 23: 12746. https://doi.org/10.3390/ijms222312746

APA Style

Wang, J., Xu, Y., Qin, C., Hu, J., Yin, J., & Guo, X. (2021). Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup. International Journal of Molecular Sciences, 22(23), 12746. https://doi.org/10.3390/ijms222312746

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Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup

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