Enhanced Platelet-Rich Plasma (ePRP) Stimulates Wound Healing through Effects on Metabolic Reprogramming in Fibroblasts
Abstract
:1. Introduction
2. Results
2.1. ePRP Stimulates Wound Healing by Releasing Critical Growth Factors
2.2. ePRP Triggers a Metabolic Reprogramming in Fibroblasts
2.3. ePRP Impedes the Mitochondrial Activities in Fibroblasts
2.4. SIRT1 Activation by ePRP Halts Senescence-Associated Features in Fibroblasts
2.5. Glycolytic Inhibition Abolishes the ePRP-Stimulated Wound Healing Capacity of Fibroblasts
3. Discussion
4. Materials and Methods
4.1. Preparation of Enhanced Platelet-Rich Plasma (ePRP)
4.2. Human Growth Factor Cytokine Array
4.3. Cell Culture
4.4. ePRP Treatments
4.5. Cell Proliferation Assay
4.6. Wound Healing Migration Assay
4.7. RNA Isolation and Real-Time RT-PCR
4.8. Measurement of the Intracellular ATP Content
4.9. Flow Cytometry Analysis
4.10. Senescence β-Galactosidase Staining
4.11. Metabolic Flux Assays
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Weng, H.-P.; Cheng, Y.-Y.; Lee, H.-L.; Hsu, T.-Y.; Chang, Y.-T.; Shen, Y.-A. Enhanced Platelet-Rich Plasma (ePRP) Stimulates Wound Healing through Effects on Metabolic Reprogramming in Fibroblasts. Int. J. Mol. Sci. 2021, 22, 12623. https://doi.org/10.3390/ijms222312623
Weng H-P, Cheng Y-Y, Lee H-L, Hsu T-Y, Chang Y-T, Shen Y-A. Enhanced Platelet-Rich Plasma (ePRP) Stimulates Wound Healing through Effects on Metabolic Reprogramming in Fibroblasts. International Journal of Molecular Sciences. 2021; 22(23):12623. https://doi.org/10.3390/ijms222312623
Chicago/Turabian StyleWeng, Hsin-Pei, Yuan-Yang Cheng, Hsin-Lun Lee, Tai-Yi Hsu, Yu-Tang Chang, and Yao-An Shen. 2021. "Enhanced Platelet-Rich Plasma (ePRP) Stimulates Wound Healing through Effects on Metabolic Reprogramming in Fibroblasts" International Journal of Molecular Sciences 22, no. 23: 12623. https://doi.org/10.3390/ijms222312623
APA StyleWeng, H. -P., Cheng, Y. -Y., Lee, H. -L., Hsu, T. -Y., Chang, Y. -T., & Shen, Y. -A. (2021). Enhanced Platelet-Rich Plasma (ePRP) Stimulates Wound Healing through Effects on Metabolic Reprogramming in Fibroblasts. International Journal of Molecular Sciences, 22(23), 12623. https://doi.org/10.3390/ijms222312623