Exploring the Impact of the Microbiome on Neuroactive Steroid Levels in Germ-Free Animals
Abstract
:1. Introduction
2. Results
2.1. Assessment of Neuroactive Steroid Levels in Plasma
2.2. Assessment of Neuroactive Steroid Levels in Brain Areas
2.2.1. Hippocampus
2.2.2. Cerebellum and Cerebral Cortex
2.2.3. Hypothalamus
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Tissue Collection
4.3. Reagents and Chemicals
4.4. Liquid Chromatography–Tandem Mass Spectrometry Analysis
4.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Diviccaro, S.; Caputi, V.; Cioffi, L.; Giatti, S.; Lyte, J.M.; Caruso, D.; O’Mahony, S.M.; Melcangi, R.C. Exploring the Impact of the Microbiome on Neuroactive Steroid Levels in Germ-Free Animals. Int. J. Mol. Sci. 2021, 22, 12551. https://doi.org/10.3390/ijms222212551
Diviccaro S, Caputi V, Cioffi L, Giatti S, Lyte JM, Caruso D, O’Mahony SM, Melcangi RC. Exploring the Impact of the Microbiome on Neuroactive Steroid Levels in Germ-Free Animals. International Journal of Molecular Sciences. 2021; 22(22):12551. https://doi.org/10.3390/ijms222212551
Chicago/Turabian StyleDiviccaro, Silvia, Valentina Caputi, Lucia Cioffi, Silvia Giatti, Joshua M. Lyte, Donatella Caruso, Siobhain M. O’Mahony, and Roberto Cosimo Melcangi. 2021. "Exploring the Impact of the Microbiome on Neuroactive Steroid Levels in Germ-Free Animals" International Journal of Molecular Sciences 22, no. 22: 12551. https://doi.org/10.3390/ijms222212551