Garlic Volatile Diallyl Disulfide Induced Cucumber Resistance to Downy Mildew
Abstract
:1. Introduction
2. Results
2.1. DADS-Induced Cucumber Resistance to Downy Mildew
2.2. DADS Induced More ROS and Lignin Accumulations and Defense Enzymes Divergences after P. cubensis Inoculation
2.3. DADS Induced Phytohormone Changes under the Infection of Downy Mildew
2.4. DADS-Induced Significant DEGs in Cucumber to Improve its Downy Mildew Resistance
2.5. WGCNA Provides Insights into Cucumber Resistance to P. cubensis
3. Discussion
3.1. Possible PTI and ETI Defense Mechanisms
3.2. DADS Induced H2O2 and Lignin Response to P. cubensis Infection
3.3. DADS Induced GST Response to P. cubensis Infection
3.4. DADS Induced Plant Hormone Response to P. cubensis Infection
3.5. DADS Induced WRKY33 Response to P. cubensis Infection
3.6. Possible Resistance Pathways in DADS Induced Cucumber Resistance to Downy Mildew
4. Materials and Methods
4.1. Cucumber Plant and P. cubensis Preparation
4.2. DADS Treatment and P. cubensis Inoculation
4.3. Histological Observation
4.4. Biochemical Assays
4.5. RNA Extraction, cDNA Library Construction and Sequencing
4.6. Differentially Expressed Genes (DEGs) Analysis
4.7. Expression Analysis of Disease Resistance-Related Genes
4.8. Analysis of KEGG Enrichment of DEGs
4.9. Weighted Gene Co-Expression Network Analysis (WGCNA)
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Description | Module Colors | All.k within |
---|---|---|---|
CsGy4G012800 | Peroxidase | blue | 179.41 |
CsGy4G000360 | Aminotransferase | blue | 178.46 |
CsGy6G023900 | Phenylalanine ammonia-lyase | blue | 146.39 |
CsGy1G004610 | probable WRKY transcription factor 33 | pink | 12.23 |
CsGy1G004050 | probable glutathione S-transferase | blue | 104.07 |
CsGy6G010290 | indole-3-acetic acid-amido synthetase GH3.6 | tan | 39.20 |
CsGy5G008430 | abscisic acid receptor PYL4 | blue | 111.72 |
CsGy7G006240 | PR1, Cysteine-rich venom protein | turquoise | 27.60 |
CsGy1G001860 | protein EDS1L-like | cyan | 17.28 |
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Yang, F.; Wang, H.; Zhi, C.; Chen, B.; Zheng, Y.; Qiao, L.; Gao, J.; Pan, Y.; Cheng, Z. Garlic Volatile Diallyl Disulfide Induced Cucumber Resistance to Downy Mildew. Int. J. Mol. Sci. 2021, 22, 12328. https://doi.org/10.3390/ijms222212328
Yang F, Wang H, Zhi C, Chen B, Zheng Y, Qiao L, Gao J, Pan Y, Cheng Z. Garlic Volatile Diallyl Disulfide Induced Cucumber Resistance to Downy Mildew. International Journal of Molecular Sciences. 2021; 22(22):12328. https://doi.org/10.3390/ijms222212328
Chicago/Turabian StyleYang, Fan, Hui Wang, Chengchen Zhi, Birong Chen, Yujie Zheng, Lijun Qiao, Jingcao Gao, Yupeng Pan, and Zhihui Cheng. 2021. "Garlic Volatile Diallyl Disulfide Induced Cucumber Resistance to Downy Mildew" International Journal of Molecular Sciences 22, no. 22: 12328. https://doi.org/10.3390/ijms222212328