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Article

Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation

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Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, Croatia
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Mediterranean Institute for Life Sciences, 21000 Split, Croatia
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Clinic for Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
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Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
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Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
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Reproduction and Obstetrics Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
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Authors to whom correspondence should be addressed.
Academic Editors: Federica Iavarone and Andrea Urbani
Int. J. Mol. Sci. 2021, 22(21), 11678; https://doi.org/10.3390/ijms222111678
Received: 22 September 2021 / Revised: 19 October 2021 / Accepted: 25 October 2021 / Published: 28 October 2021
(This article belongs to the Special Issue Proteomics and Metabonomics for Personalised Medicine)
Herniation of the intervertebral disc (IVDH) is the most common cause of neurological and intervertebral disc degeneration-related diseases. Since the disc starts to degenerate before it can be observed by currently available diagnostic methods, there is an urgent need for novel diagnostic approaches. To identify molecular networks and pathways which may play important roles in intervertebral disc herniation, as well as to reveal the potential features which could be useful for monitoring disease progression and prognosis, multi-omics profiling, including high-resolution liquid chromatography-mass spectrometry (LC-MS)-based metabolomics and tandem mass tag (TMT)-based proteomics was performed. Cerebrospinal fluid of nine dogs with IVDH and six healthy controls were used for the analyses, and an additional five IVDH samples were used for proteomic data validation. Furthermore, multi-omics data were integrated to decipher a complex interaction between individual omics layers, leading to an improved prediction model. Together with metabolic pathways related to amino acids and lipid metabolism and coagulation cascades, our integromics prediction model identified the key features in IVDH, namely the proteins follistatin Like 1 (FSTL1), secretogranin V (SCG5), nucleobindin 1 (NUCB1), calcitonin re-ceptor-stimulating peptide 2 precursor (CRSP2) and the metabolites N-acetyl-D-glucosamine and adenine, involved in neuropathic pain, myelination, and neurotransmission and inflammatory response, respectively. Their clinical application is to be further investigated. The utilization of a novel integrative interdisciplinary approach may provide new opportunities to apply innovative diagnostic and monitoring methods as well as improve treatment strategies and personalized care for patients with degenerative spinal disorders. View Full-Text
Keywords: cerebrospinal fluid; dog; integromics; intervertebral disc herniation; metabolomics; proteomics; personalized medicine cerebrospinal fluid; dog; integromics; intervertebral disc herniation; metabolomics; proteomics; personalized medicine
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MDPI and ACS Style

Horvatić, A.; Gelemanović, A.; Pirkić, B.; Smolec, O.; Beer Ljubić, B.; Rubić, I.; Eckersall, P.D.; Mrljak, V.; McLaughlin, M.; Samardžija, M.; Lipar, M. Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation. Int. J. Mol. Sci. 2021, 22, 11678. https://doi.org/10.3390/ijms222111678

AMA Style

Horvatić A, Gelemanović A, Pirkić B, Smolec O, Beer Ljubić B, Rubić I, Eckersall PD, Mrljak V, McLaughlin M, Samardžija M, Lipar M. Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation. International Journal of Molecular Sciences. 2021; 22(21):11678. https://doi.org/10.3390/ijms222111678

Chicago/Turabian Style

Horvatić, Anita, Andrea Gelemanović, Boris Pirkić, Ozren Smolec, Blanka Beer Ljubić, Ivana Rubić, Peter D. Eckersall, Vladimir Mrljak, Mark McLaughlin, Marko Samardžija, and Marija Lipar. 2021. "Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation" International Journal of Molecular Sciences 22, no. 21: 11678. https://doi.org/10.3390/ijms222111678

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