Peimine, an Anti-Inflammatory Compound from Chinese Herbal Extracts, Modulates Muscle-Type Nicotinic Receptors
, José Antonio Encinar 2, Raúl Cobo 1, Gregorio Fernández-Ballester 2, José Manuel González-Ros 2, Isabel Ivorra 1 and Andrés Morales 1,*Round 1
Reviewer 1 Report
Authors report activities of pemine. The results are very clear. Therefore, I recommend this manuscript to be published in International Journal of Molecular Science.
Reviewer 2 Report
Manuscript ID: ijms-1418114
Type of manuscript: Article
Title: Peimine, an anti-inflammatory compound from Chinese herbal extracts, modulates muscle-type nicotinic receptors.
The authors studied the effects of Peimine (an isosteroidal alkaloid considered one of the main bioactive molecules of Fritillaria bulbs) on nicotinic acetylcholine receptors (nAChRs) in both ways the nervous system and non-neuronal tissues. The results show that Peimine acts at submicromolar concentrations as a potent modulator of nAChRs. Besides, Peimine binds to the nAChRs with high affinity at multiple sites but principally located at the transmembrane domain (TMD), inhibiting its function by different mechanisms. These results expand our understanding of the modulation of nAChRs by biomolecules with therapeutic relevance.
The study is very well-conceived, with very specific objectives, and the results are excellently presented. The discussion considers the most relevant bibliography regarding the subject and is also very well organized. Therefore, the manuscript is suitable for publication in the present form.
