Next Article in Journal
The Mandible Ameliorates Facial Allograft Rejection and Is Associated with the Development of Regulatory T Cells and Mixed Chimerism
Next Article in Special Issue
Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber
Previous Article in Journal
Anti-Kasha Behavior of 3-Hydroxyflavone and Its Derivatives
Previous Article in Special Issue
Isolation and Characterization of Barley (Hordeum vulgare) Extracellular Vesicles to Assess Their Role in RNA Spray-Based Crop Protection
Article

Distinct Evolutionary Profiles and Functions of microRNA156 and microRNA529 in Land Plants

1
Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China
2
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
3
Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
4
Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, USA
*
Authors to whom correspondence should be addressed.
Academic Editors: Ivan Minkov and Vesselin Baev
Int. J. Mol. Sci. 2021, 22(20), 11100; https://doi.org/10.3390/ijms222011100
Received: 26 July 2021 / Revised: 23 September 2021 / Accepted: 11 October 2021 / Published: 14 October 2021
(This article belongs to the Special Issue Plant Non-coding RNAs)
MicroRNA156 (miR156) and miR529 have high sequence similarity and recognize overlapping sites in the same target genes, SQUAMOSA promoter binding protein-like (SPL or SBP box) genes, making it difficult to accurately distinguish their roles in regulatory networks that affect numerous biological functions. Here, we collected data about miR156 and miR529 family members from representative land plants and performed sequence comparisons, phylogenetic analysis, small RNA sequencing, and parallel analysis of RNA ends (PARE) analysis to dissect their evolutionary and functional differences. Although miR156 and miR529 are highly similar, there are differences in their mismatch-sensitive regions, which are essential for target recognition. In land plants, miR156 precursors are conserved mainly within the hairpin region, whereas miR529 precursors are conserved outside the hairpin region, including both the 5’ and 3’ arms. Phylogenetic analysis showed that MIR156 and MIR529 evolved independently, through divergent evolutionary patterns. The two genes also exhibit different expression patterns, with MIR529 preferentially expressed in reproductive tissues and MIR156 in other tissues. PARE analysis revealed that miR156 and miR529 possess specific targets in addition to common targets in maize, pointing to functional differences between them. Based on our findings, we developed a method for the rapid identification of miR529 and miR156 family members and uncovered the evolutionary divergence of these families, providing insights into their different regulatory roles in plant growth and development. View Full-Text
Keywords: miR156; miR529; SQUAMOSA promoter binding protein-like genes; evolution; land plants miR156; miR529; SQUAMOSA promoter binding protein-like genes; evolution; land plants
Show Figures

Figure 1

MDPI and ACS Style

Xie, Q.; Wang, X.; He, J.; Lan, T.; Zheng, J.; Li, Y.; Pan, J.; Lin, L.; Zhao, J.; Li, J.; Yu, Y.; Mo, B.; Chen, X.; Gao, L.; Liu, L. Distinct Evolutionary Profiles and Functions of microRNA156 and microRNA529 in Land Plants. Int. J. Mol. Sci. 2021, 22, 11100. https://doi.org/10.3390/ijms222011100

AMA Style

Xie Q, Wang X, He J, Lan T, Zheng J, Li Y, Pan J, Lin L, Zhao J, Li J, Yu Y, Mo B, Chen X, Gao L, Liu L. Distinct Evolutionary Profiles and Functions of microRNA156 and microRNA529 in Land Plants. International Journal of Molecular Sciences. 2021; 22(20):11100. https://doi.org/10.3390/ijms222011100

Chicago/Turabian Style

Xie, Qi, Xufeng Wang, Juan He, Ting Lan, Jiayu Zheng, Yupeng Li, Jinkang Pan, Ling Lin, Junyi Zhao, Jing Li, Yu Yu, Beixin Mo, Xuemei Chen, Lei Gao, and Lin Liu. 2021. "Distinct Evolutionary Profiles and Functions of microRNA156 and microRNA529 in Land Plants" International Journal of Molecular Sciences 22, no. 20: 11100. https://doi.org/10.3390/ijms222011100

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop