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Article

Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii

by
Yapei Zhao
†,
Tian Hu
†,
Ruiqi Liu
,
Zhiqiang Hao
,
Guoyan Liang
and
Guanglin Li
*
Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, Shaanxi, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2021, 22(2), 605; https://doi.org/10.3390/ijms22020605
Submission received: 12 December 2020 / Revised: 6 January 2021 / Accepted: 7 January 2021 / Published: 9 January 2021
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Selaginella moellendorffii is a lycophyte, a member of an ancient vascular plant lineage. Two distinct types of terpene synthase (TPS) genes were identified from this species, including S. moellendorffii TPS genes (SmTPSs) and S. moellendorffii microbial TPS-like genes (SmMTPSLs). The goal of this study was to investigate the biochemical functions of SmMTPSLs. Here, eight full-length SmMTPSL genes (SmMTPSL5, -15, -19, -23, -33, -37, -46, and -47) were functionally characterized from S. moellendorffii. Escherichia coli-expressed recombinant SmMTPSLs were tested for monoterpenes synthase and sesquiterpenes synthase activities. These enzymatic products were typical monoterpenes and sesquiterpenes that have been previous shown to be generated by typical plant TPSs when provided with geranyl diphosphate (GPP) and farnesyl diphosphate (FPP) as the substrates. Meanwhile, SmMTPSL23, -33, and -37 were up-regulated when induced by alamethicin (ALA) and methyl jasmonate (MeJA), suggesting a role for these genes in plants response to abiotic stresses. Furthermore, this study pointed out that the terpenoids products of SmMTPSL23, -33, and -37 have an antibacterial effect on Pseudomonas syringae pv. tomato DC3000 and Staphylococcus aureus. Taken together, these results provide more information about the catalytic and biochemical function of SmMTPSLs in S. moellendorffii plants.
Keywords: lycophyte; SmMTPSLs; stress physiology; GC-MS; antibacterial lycophyte; SmMTPSLs; stress physiology; GC-MS; antibacterial

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MDPI and ACS Style

Zhao, Y.; Hu, T.; Liu, R.; Hao, Z.; Liang, G.; Li, G. Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii. Int. J. Mol. Sci. 2021, 22, 605. https://doi.org/10.3390/ijms22020605

AMA Style

Zhao Y, Hu T, Liu R, Hao Z, Liang G, Li G. Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii. International Journal of Molecular Sciences. 2021; 22(2):605. https://doi.org/10.3390/ijms22020605

Chicago/Turabian Style

Zhao, Yapei, Tian Hu, Ruiqi Liu, Zhiqiang Hao, Guoyan Liang, and Guanglin Li. 2021. "Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii" International Journal of Molecular Sciences 22, no. 2: 605. https://doi.org/10.3390/ijms22020605

APA Style

Zhao, Y., Hu, T., Liu, R., Hao, Z., Liang, G., & Li, G. (2021). Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii. International Journal of Molecular Sciences, 22(2), 605. https://doi.org/10.3390/ijms22020605

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