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Calmodulin as Ca2+-Dependent Interactor of FTO Dioxygenase

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland
Center of Translational Medicine, Warsaw University of Life Sciences, Nowoursynowska 100, 02-797 Warsaw, Poland
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Suzanne F. Scarlata and Eui-Bae Jeung
Int. J. Mol. Sci. 2021, 22(19), 10869;
Received: 10 September 2021 / Revised: 24 September 2021 / Accepted: 6 October 2021 / Published: 8 October 2021
(This article belongs to the Section Molecular Toxicology)
FTO is an N6-methyladenosine demethylase removing methyl groups from nucleic acids. Several studies indicate the creation of FTO complexes with other proteins. Here, we looked for regulatory proteins recognizing parts of the FTO dioxygenase region. In the Calmodulin (CaM) Target Database, we found the FTO C-domain potentially binding CaM, and we proved this finding experimentally. The interaction was Ca2+-dependent but independent on FTO phosphorylation. We found that FTO–CaM interaction essentially influences calcium-binding loops in CaM, indicating the presence of two peptide populations—exchanging as CaM alone and differently, suggesting that only one part of CaM interacts with FTO, and the other one reminds free. The modeling of FTO–CaM interaction showed its stable structure when the half of the CaM molecule saturated with Ca2+ interacts with the FTO C-domain, whereas the other part is disconnected. The presented data indicate calmodulin as a new FTO interactor and support engagement of the FTO protein in calcium signaling pathways. View Full-Text
Keywords: FTO; calmodulin; calcium; MST; HDX FTO; calmodulin; calcium; MST; HDX
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MDPI and ACS Style

Marcinkowski, M.; Pilžys, T.; Garbicz, D.; Piwowarski, J.; Przygońska, K.; Winiewska-Szajewska, M.; Ferenc, K.; Skorobogatov, O.; Poznański, J.; Grzesiuk, E. Calmodulin as Ca2+-Dependent Interactor of FTO Dioxygenase. Int. J. Mol. Sci. 2021, 22, 10869.

AMA Style

Marcinkowski M, Pilžys T, Garbicz D, Piwowarski J, Przygońska K, Winiewska-Szajewska M, Ferenc K, Skorobogatov O, Poznański J, Grzesiuk E. Calmodulin as Ca2+-Dependent Interactor of FTO Dioxygenase. International Journal of Molecular Sciences. 2021; 22(19):10869.

Chicago/Turabian Style

Marcinkowski, Michał, Tomaš Pilžys, Damian Garbicz, Jan Piwowarski, Kaja Przygońska, Maria Winiewska-Szajewska, Karolina Ferenc, Oleksandr Skorobogatov, Jarosław Poznański, and Elżbieta Grzesiuk. 2021. "Calmodulin as Ca2+-Dependent Interactor of FTO Dioxygenase" International Journal of Molecular Sciences 22, no. 19: 10869.

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