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Article

Tissue Sampling and Homogenization in the Sub-Microliter Scale with a Nanosecond Infrared Laser (NIRL) for Mass Spectrometric Proteomics

1
Section/Core Facility Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
2
Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Nagib Ahsan
Int. J. Mol. Sci. 2021, 22(19), 10833; https://doi.org/10.3390/ijms221910833
Received: 25 August 2021 / Revised: 30 September 2021 / Accepted: 2 October 2021 / Published: 7 October 2021
It was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient for sampling and homogenizing biological tissue. In this study we utilized a tunable nanosecond infrared laser (NIRL) for tissue sampling and homogenization with subsequent liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis for mass spectrometric proteomics. For the first time, laser sampling was performed with murine spleen and colon tissue. An ablation volume of 1.1 × 1.1 × 0.4 mm³ (approximately 0.5 µL) was determined with optical coherence tomography (OCT). The results of bottom-up proteomics revealed proteins with significant abundance differences for both tissue types, which are in accordance with the corresponding data of the Human Protein Atlas. The results demonstrate that tissue sampling and homogenization of small tissue volumes less than 1 µL for subsequent mass spectrometric proteomics is feasible with a NIRL. View Full-Text
Keywords: tissue sampling; tissue homogenization; nanosecond infrared laser; laser ablation; proteomics; mass spectrometry tissue sampling; tissue homogenization; nanosecond infrared laser; laser ablation; proteomics; mass spectrometry
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MDPI and ACS Style

Hahn, J.; Moritz, M.; Voß, H.; Pelczar, P.; Huber, S.; Schlüter, H. Tissue Sampling and Homogenization in the Sub-Microliter Scale with a Nanosecond Infrared Laser (NIRL) for Mass Spectrometric Proteomics. Int. J. Mol. Sci. 2021, 22, 10833. https://doi.org/10.3390/ijms221910833

AMA Style

Hahn J, Moritz M, Voß H, Pelczar P, Huber S, Schlüter H. Tissue Sampling and Homogenization in the Sub-Microliter Scale with a Nanosecond Infrared Laser (NIRL) for Mass Spectrometric Proteomics. International Journal of Molecular Sciences. 2021; 22(19):10833. https://doi.org/10.3390/ijms221910833

Chicago/Turabian Style

Hahn, Jan, Manuela Moritz, Hannah Voß, Penelope Pelczar, Samuel Huber, and Hartmut Schlüter. 2021. "Tissue Sampling and Homogenization in the Sub-Microliter Scale with a Nanosecond Infrared Laser (NIRL) for Mass Spectrometric Proteomics" International Journal of Molecular Sciences 22, no. 19: 10833. https://doi.org/10.3390/ijms221910833

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