Effects of Growth Hormone Receptor Ablation in Corticotropin-Releasing Hormone Cells
Abstract
:1. Introduction
2. Results
2.1. Distribution of CRH Neurons That Are Responsive to GH
2.2. Reduced Energy Expenditure in CRH GHR KO Female Mice
2.3. GHR Ablation in CRH Cells Does Not Affect Glucose Homeostasis and Counterregulatory Response
2.4. Ghrelin-Induced Food Intake Is Normal in CRH GHR KO Mice
2.5. GHR Ablation in CRH Cells Does Not Affect Circadian or Restraint Stress-Induced Corticosterone Secretion
2.6. CRH GHR KO Mice Exhibit Normal Activation of PVH Neurons after Restraint Stress
2.7. CRH GHR KO Mice Exhibit Normal Anxiety
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Detection of GH Responsive neurons
4.3. Metabolic Measurements
4.4. Evaluation of Ghrelin-Induced Food Intake
4.5. Evaluation of Circadian and Restraint-Stress-Induced Corticosterone Secretion
4.6. Analysis of Stress-Induced Fos Expression
4.7. Open Field and Elevated Plus Maze Tests
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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dos Santos, W.O.; Gusmao, D.O.; Wasinski, F.; List, E.O.; Kopchick, J.J.; Donato Jr., J. Effects of Growth Hormone Receptor Ablation in Corticotropin-Releasing Hormone Cells. Int. J. Mol. Sci. 2021, 22, 9908. https://doi.org/10.3390/ijms22189908
dos Santos WO, Gusmao DO, Wasinski F, List EO, Kopchick JJ, Donato Jr. J. Effects of Growth Hormone Receptor Ablation in Corticotropin-Releasing Hormone Cells. International Journal of Molecular Sciences. 2021; 22(18):9908. https://doi.org/10.3390/ijms22189908
Chicago/Turabian Styledos Santos, Willian O., Daniela O. Gusmao, Frederick Wasinski, Edward O. List, John J. Kopchick, and Jose Donato Jr. 2021. "Effects of Growth Hormone Receptor Ablation in Corticotropin-Releasing Hormone Cells" International Journal of Molecular Sciences 22, no. 18: 9908. https://doi.org/10.3390/ijms22189908