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Article

Identification of Abundant and Functional dodecaRNAs (doRNAs) Derived from Ribosomal RNA

1
CHU de Québec Research Center/CHUL Pavilion—Université Laval, 2705 boulevard Laurier, Quebec City, QC G1V 4G2, Canada
2
Department of Microbiology, Infectious Diseases and Immunology, Université Laval, Quebec City, QC G1V 4G2, Canada
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Faculty of Medicine, Université Laval, Quebec City, QC G1V 0A6, Canada
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Department of Molecular Medicine, Université Laval, Quebec City, QC G1V 4G2, Canada
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Department of Surgery, Université Laval, Quebec City, QC G1R 2J6, Canada
*
Author to whom correspondence should be addressed.
Deceased.
Academic Editors: Francisco J. Enguita and John Mattick
Int. J. Mol. Sci. 2021, 22(18), 9757; https://doi.org/10.3390/ijms22189757
Received: 4 August 2021 / Revised: 2 September 2021 / Accepted: 5 September 2021 / Published: 9 September 2021
(This article belongs to the Special Issue RNA Regulatory Networks 2.0)
Using a modified RNA-sequencing (RNA-seq) approach, we discovered a new family of unusually short RNAs mapping to ribosomal RNA 5.8S, which we named dodecaRNAs (doRNAs), according to the number of core nucleotides (12 nt) their members contain. Using a new quantitative detection method that we developed, we confirmed our RNA-seq data and determined that the minimal core doRNA sequence and its 13-nt variant C-doRNA (doRNA with a 5′ Cytosine) are the two most abundant doRNAs, which, together, may outnumber microRNAs. The C-doRNA/doRNA ratio is stable within species but differed between species. doRNA and C-doRNA are mainly cytoplasmic and interact with heterogeneous nuclear ribonucleoproteins (hnRNP) A0, A1 and A2B1, but not Argonaute 2. Reporter gene activity assays suggest that C-doRNA may function as a regulator of Annexin II receptor (AXIIR) expression. doRNAs are differentially expressed in prostate cancer cells/tissues and may control cell migration. These findings suggest that unusually short RNAs may be more abundant and important than previously thought. View Full-Text
Keywords: RNA sequencing; small RNA; non-coding RNA; RT-qPCR; 5.8S rRNA RNA sequencing; small RNA; non-coding RNA; RT-qPCR; 5.8S rRNA
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MDPI and ACS Style

Lambert, M.; Benmoussa, A.; Diallo, I.; Ouellet-Boutin, K.; Dorval, V.; Majeau, N.; Joly-Beauparlant, C.; Droit, A.; Bergeron, A.; Têtu, B.; Fradet, Y.; Pouliot, F.; Provost, P. Identification of Abundant and Functional dodecaRNAs (doRNAs) Derived from Ribosomal RNA. Int. J. Mol. Sci. 2021, 22, 9757. https://doi.org/10.3390/ijms22189757

AMA Style

Lambert M, Benmoussa A, Diallo I, Ouellet-Boutin K, Dorval V, Majeau N, Joly-Beauparlant C, Droit A, Bergeron A, Têtu B, Fradet Y, Pouliot F, Provost P. Identification of Abundant and Functional dodecaRNAs (doRNAs) Derived from Ribosomal RNA. International Journal of Molecular Sciences. 2021; 22(18):9757. https://doi.org/10.3390/ijms22189757

Chicago/Turabian Style

Lambert, Marine, Abderrahim Benmoussa, Idrissa Diallo, Katheryn Ouellet-Boutin, Véronique Dorval, Nathalie Majeau, Charles Joly-Beauparlant, Arnaud Droit, Alain Bergeron, Bernard Têtu, Yves Fradet, Frédéric Pouliot, and Patrick Provost. 2021. "Identification of Abundant and Functional dodecaRNAs (doRNAs) Derived from Ribosomal RNA" International Journal of Molecular Sciences 22, no. 18: 9757. https://doi.org/10.3390/ijms22189757

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