Proteomic and Biochemical Analyses of the Mechanism of Tolerance in Mutant Soybean Responding to Flooding Stress
Abstract
:1. Introduction
2. Results
2.1. Morphological Analysis of Mutant Soybean under Flooding Stress
2.2. Identification and Functional Investigation of Proteins in Mutant Soybean under Flooding Stress
2.3. Immunoblot Analysis of Proteins Involved in Endoplasmic Reticulum in Mutant Soybean under Flooding Stress
2.4. Alcohol Dehydrogenase (ADH) Accumulation and Activity in Mutant Soybean under Flooding Stress
2.5. Evans-Blue Staining with Mutant Soybean under Flooding Stress
3. Discussion
3.1. Mutation in Flooding-Tolerant Soybean Has Positive Effects on the Growth of Plant under Flooding Stress
3.2. Regulation of Alcohol-Formation Pathway Is Related to the Mechanism of Flooding Tolerance
3.3. Glycoprotein Folding Is Related to the Mechanism of Flooding Tolerance
3.4. Cell Death in Soybean Root Is Related to Flooding Tolerance
4. Materials and Methods
4.1. Plant Material and Experimental Design
4.2. Protein Extraction, Protein Enrichment, Reduction, Alkylation, and Digestion
4.3. Protein Identification Using Nano-Liquid Chromatography Mass Spectrometry
4.4. Mass Spectrometry Data Analysis
4.5. Differential Analysis of Proteins Using Mass Spectrometry Data
4.6. Bioinformatic Analyses of Protein Functional Categorization
4.7. Immunoblot Analysis
4.8. ADH Activity Assay
4.9. Evaluation of Cell Death Using Evans-Blue Dye
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Komatsu, S.; Yamaguchi, H.; Hitachi, K.; Tsuchida, K.; Kono, Y.; Nishimura, M. Proteomic and Biochemical Analyses of the Mechanism of Tolerance in Mutant Soybean Responding to Flooding Stress. Int. J. Mol. Sci. 2021, 22, 9046. https://doi.org/10.3390/ijms22169046
Komatsu S, Yamaguchi H, Hitachi K, Tsuchida K, Kono Y, Nishimura M. Proteomic and Biochemical Analyses of the Mechanism of Tolerance in Mutant Soybean Responding to Flooding Stress. International Journal of Molecular Sciences. 2021; 22(16):9046. https://doi.org/10.3390/ijms22169046
Chicago/Turabian StyleKomatsu, Setsuko, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida, Yuhi Kono, and Minoru Nishimura. 2021. "Proteomic and Biochemical Analyses of the Mechanism of Tolerance in Mutant Soybean Responding to Flooding Stress" International Journal of Molecular Sciences 22, no. 16: 9046. https://doi.org/10.3390/ijms22169046