The Arabidopsis HY2 Gene Acts as a Positive Regulator of NaCl Signaling during Seed Germination
Abstract
:1. Introduction
2. Results
2.1. Disruption of HY2 Reduces, and Overexpression of HY2 Enhances, NaCl Sensitivity during Seed Germination
2.2. Quantitative Proteomics Analysis of col4 and hy2 Mutant under SALT Stress
2.3. Function Analysis of Accurate Protein Quantification
2.4. Function Analysis of CRPs
2.5. HY2-Specific Regulated Pathway Relating to the Salt Stress Response
2.6. Disruption of HY2-Altered Expression of a Set of Stress-Responsive Genes
2.7. Interaction Network of HY2-Specific DRPs
3. Discussion
4. Materials and Methods
4.1. Plasmid Construction
4.2. Plant Materials and Growth Conditions
4.3. Salt Sensitivity Assay
4.4. LUC Activity Recordings
4.5. QPCR Assay
4.6. Tandem Mass Tags (TMT)-Based Proteomics Analysis
4.7. GO Enrichment Analysis
4.8. Interaction Network Analysis
4.9. Quantification and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Piao, M.; Zou, J.; Li, Z.; Zhang, J.; Yang, L.; Yao, N.; Li, Y.; Li, Y.; Tang, H.; Zhang, L.; et al. The Arabidopsis HY2 Gene Acts as a Positive Regulator of NaCl Signaling during Seed Germination. Int. J. Mol. Sci. 2021, 22, 9009. https://doi.org/10.3390/ijms22169009
Piao M, Zou J, Li Z, Zhang J, Yang L, Yao N, Li Y, Li Y, Tang H, Zhang L, et al. The Arabidopsis HY2 Gene Acts as a Positive Regulator of NaCl Signaling during Seed Germination. International Journal of Molecular Sciences. 2021; 22(16):9009. https://doi.org/10.3390/ijms22169009
Chicago/Turabian StylePiao, Mingxin, Jinpeng Zou, Zhifang Li, Junchuan Zhang, Liang Yang, Nan Yao, Yuhong Li, Yaxing Li, Haohao Tang, Li Zhang, and et al. 2021. "The Arabidopsis HY2 Gene Acts as a Positive Regulator of NaCl Signaling during Seed Germination" International Journal of Molecular Sciences 22, no. 16: 9009. https://doi.org/10.3390/ijms22169009