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Article

Enzymatic Decontamination of G-Type, V-Type and Novichok Nerve Agents

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Gene&GreenTK, 19–21 Boulevard Jean Moulin, 13005 Marseille, France
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TNO Department CBRN Protection, Lange Kleiweg 137, 2288 GJ Rijswijk, The Netherlands
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Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Aix-Marseille Université, 13005 Marseille, France
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Institut de Recherche pour le Développement (IRD), Assistance Publique Hôpitaux de Marseille (APHM), Unité Microbe Evolution Phylogénie et Infection (MEPHI), 13005 Marseille, France
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Authors to whom correspondence should be addressed.
Academic Editor: Jan Korabecny
Int. J. Mol. Sci. 2021, 22(15), 8152; https://doi.org/10.3390/ijms22158152
Received: 6 July 2021 / Revised: 23 July 2021 / Accepted: 26 July 2021 / Published: 29 July 2021
(This article belongs to the Section Biochemistry)
Organophosphorus nerve agents (OPNAs) are highly toxic compounds inhibiting cholinergic enzymes in the central and autonomic nervous systems and neuromuscular junctions, causing severe intoxications in humans. Medical countermeasures and efficient decontamination solutions are needed to counteract the toxicity of a wide spectrum of harmful OPNAs including G, V and Novichok agents. Here, we describe the use of engineered OPNA-degrading enzymes for the degradation of various toxic agents including insecticides, a series of OPNA surrogates, as well as real chemical warfare agents (cyclosarin, sarin, soman, tabun, VX, A230, A232, A234). We demonstrate that only two enzymes can degrade most of these molecules at high concentrations (25 mM) in less than 5 min. Using surface assays adapted from NATO AEP-65 guidelines, we further show that enzyme-based solutions can decontaminate 97.6% and 99.4% of 10 g∙m2 of soman- and VX-contaminated surfaces, respectively. Finally, we demonstrate that these enzymes can degrade ethyl-paraoxon down to sub-inhibitory concentrations of acetylcholinesterase, confirming their efficacy from high to micromolar doses. View Full-Text
Keywords: decontamination; phosphotriesterase; nerve agents; organophosphorus; Novichok; sarin; VX decontamination; phosphotriesterase; nerve agents; organophosphorus; Novichok; sarin; VX
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MDPI and ACS Style

Jacquet, P.; Rémy, B.; Bross, R.P.T.; van Grol, M.; Gaucher, F.; Chabrière, E.; de Koning, M.C.; Daudé, D. Enzymatic Decontamination of G-Type, V-Type and Novichok Nerve Agents. Int. J. Mol. Sci. 2021, 22, 8152. https://doi.org/10.3390/ijms22158152

AMA Style

Jacquet P, Rémy B, Bross RPT, van Grol M, Gaucher F, Chabrière E, de Koning MC, Daudé D. Enzymatic Decontamination of G-Type, V-Type and Novichok Nerve Agents. International Journal of Molecular Sciences. 2021; 22(15):8152. https://doi.org/10.3390/ijms22158152

Chicago/Turabian Style

Jacquet, Pauline, Benjamin Rémy, Rowdy P.T. Bross, Marco van Grol, Floriane Gaucher, Eric Chabrière, Martijn C. de Koning, and David Daudé. 2021. "Enzymatic Decontamination of G-Type, V-Type and Novichok Nerve Agents" International Journal of Molecular Sciences 22, no. 15: 8152. https://doi.org/10.3390/ijms22158152

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