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Article

SERR Spectroelectrochemistry as a Guide for Rational Design of DyP-Based Bioelectronics Devices

1
Instituto de Tecnologia Química e Biológica António Xavier, Universidade NOVA de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
2
Gecco Biotech, Nijenborgh 4, 9747AG Groningen, The Netherlands
3
Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Yasushi Sugano
Int. J. Mol. Sci. 2021, 22(15), 7998; https://doi.org/10.3390/ijms22157998
Received: 28 June 2021 / Revised: 16 July 2021 / Accepted: 22 July 2021 / Published: 27 July 2021
(This article belongs to the Special Issue Universe of DyP-type Peroxidase)
Immobilised dye-decolorizing peroxidases (DyPs) are promising biocatalysts for the development of biotechnological devices such as biosensors for the detection of H2O2. To this end, these enzymes have to preserve native, solution properties upon immobilisation on the electrode surface. In this work, DyPs from Cellulomonas bogoriensis (CboDyP), Streptomyces coelicolor (ScoDyP) and Thermobifida fusca (TfuDyP) are immobilised on biocompatible silver electrodes functionalized with alkanethiols. Their structural, redox and catalytic properties upon immobilisation are evaluated by surface-enhanced resonance Raman (SERR) spectroelectrochemistry and cyclic voltammetry. Among the studied electrode/DyP constructs, only CboDyP shows preserved native structure upon attachment to the electrode. However, a comparison of the redox potentials of the enzyme in solution and immobilised states reveals a large discrepancy, and the enzyme shows no electrocatalytic activity in the presence of H2O2. While some immobilised DyPs outperform existing peroxidase-based biosensors, others fail to fulfil the essential requirements that guarantee their applicability in the immobilised state. The capacity of SERR spectroelectrochemistry for fast screening of the performance of immobilised heme enzymes places it in the front-line of experimental approaches that can advance the search for promising DyP candidates. View Full-Text
Keywords: DyP; SERR spectroelectrochemistry; immobilised enzymes; 3rd generation biosensors DyP; SERR spectroelectrochemistry; immobilised enzymes; 3rd generation biosensors
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MDPI and ACS Style

Zuccarello, L.; Barbosa, C.; Galdino, E.; Lončar, N.; Silveira, C.M.; Fraaije, M.W.; Todorovic, S. SERR Spectroelectrochemistry as a Guide for Rational Design of DyP-Based Bioelectronics Devices. Int. J. Mol. Sci. 2021, 22, 7998. https://doi.org/10.3390/ijms22157998

AMA Style

Zuccarello L, Barbosa C, Galdino E, Lončar N, Silveira CM, Fraaije MW, Todorovic S. SERR Spectroelectrochemistry as a Guide for Rational Design of DyP-Based Bioelectronics Devices. International Journal of Molecular Sciences. 2021; 22(15):7998. https://doi.org/10.3390/ijms22157998

Chicago/Turabian Style

Zuccarello, Lidia, Catarina Barbosa, Edilson Galdino, Nikola Lončar, Célia M. Silveira, Marco W. Fraaije, and Smilja Todorovic. 2021. "SERR Spectroelectrochemistry as a Guide for Rational Design of DyP-Based Bioelectronics Devices" International Journal of Molecular Sciences 22, no. 15: 7998. https://doi.org/10.3390/ijms22157998

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