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Article

Angulin-1 (LSR) Affects Paracellular Water Transport, However Only in Tight Epithelial Cells

Clinical Physiology/Nutritional Medicine, Medical Department, Division of Gastroenterology, Infectiology and Rheumatology, Charité—Universitätsmedizin Berlin, 12203 Berlin, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Masoud Jelokhani-Niaraki
Int. J. Mol. Sci. 2021, 22(15), 7827; https://doi.org/10.3390/ijms22157827
Received: 31 May 2021 / Revised: 6 July 2021 / Accepted: 20 July 2021 / Published: 22 July 2021
(This article belongs to the Special Issue Membrane Proteins: Structure, Function and Motion)
Water transport in epithelia occurs transcellularly (aquaporins) and paracellularly (claudin-2, claudin-15). Recently, we showed that downregulated tricellulin, a protein of the tricellular tight junction (tTJ, the site where three epithelial cells meet), increased transepithelial water flux. We now check the hypothesis that another tTJ-associated protein, angulin-1 (alias lipolysis-stimulated lipoprotein receptor, LSR) is a direct negative actuator of tTJ water permeability depending on the tightness of the epithelium. For this, a tight and an intermediate-tight epithelial cell line, MDCK C7 and HT-29/B6, were stably transfected with CRISPR/Cas9 and single-guide RNA targeting angulin-1 and morphologically and functionally characterized. Water flux induced by an osmotic gradient using 4-kDa dextran caused water flux to increase in angulin-1 KO clones in MDCK C7 cells, but not in HT-29/B6 cells. In addition, we found that water permeability in HT-29/B6 cells was not modified after either angulin-1 knockout or tricellulin knockdown, which may be related to the presence of other pathways, which reduce the impact of the tTJ pathway. In conclusion, modulation of the tTJ by knockout or knockdown of tTJ proteins affects ion and macromolecule permeability in tight and intermediate-tight epithelial cell lines, while the transepithelial water permeability was affected only in tight cell lines. View Full-Text
Keywords: angulin-1; LSR; tricellulin; tricellular tight junction; paracellular water transport; tight epithelium; MDCK C7 cells; intermediate-tight epithelium; HT-29/B6 cells angulin-1; LSR; tricellulin; tricellular tight junction; paracellular water transport; tight epithelium; MDCK C7 cells; intermediate-tight epithelium; HT-29/B6 cells
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MDPI and ACS Style

Ayala-Torres, C.; Krug, S.M.; Rosenthal, R.; Fromm, M. Angulin-1 (LSR) Affects Paracellular Water Transport, However Only in Tight Epithelial Cells. Int. J. Mol. Sci. 2021, 22, 7827. https://doi.org/10.3390/ijms22157827

AMA Style

Ayala-Torres C, Krug SM, Rosenthal R, Fromm M. Angulin-1 (LSR) Affects Paracellular Water Transport, However Only in Tight Epithelial Cells. International Journal of Molecular Sciences. 2021; 22(15):7827. https://doi.org/10.3390/ijms22157827

Chicago/Turabian Style

Ayala-Torres, Carlos, Susanne M. Krug, Rita Rosenthal, and Michael Fromm. 2021. "Angulin-1 (LSR) Affects Paracellular Water Transport, However Only in Tight Epithelial Cells" International Journal of Molecular Sciences 22, no. 15: 7827. https://doi.org/10.3390/ijms22157827

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