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Volume 22
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10.3390/ijms22147464
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Article

Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation

by
Nadja Kupper
,
Elisabeth Pritz
,
Monika Siwetz
,
Jacqueline Guettler
and
Berthold Huppertz
*
Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010 Graz, Austria
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(14), 7464; https://doi.org/10.3390/ijms22147464
Submission received: 25 June 2021 / Accepted: 9 July 2021 / Published: 12 July 2021
(This article belongs to the Special Issue Placental Related Disorders of Pregnancy)
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Abstract

During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the conditions may represent the in utero environment. However, static placental villous explant culture dramatically differs from the in vivo situation. Thus, we established a flow culture system for placental villous explants and compared commonly used static cultured tissue to flow cultured tissue using transmission and scanning electron microscopy, immunohistochemistry, and lactate dehydrogenase (LDH) and human chorionic gonadotropin (hCG) measurements. The data revealed a better structural and biochemical integrity of flow cultured tissue compared to static cultured tissue. Thus, this new flow system can be used to simulate the blood flow from the mother to the placenta and back in the most native-like in vitro system so far and thus can enable novel study designs.
Keywords: flow culture system; placenta; explant culture under flow flow culture system; placenta; explant culture under flow

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MDPI and ACS Style

Kupper, N.; Pritz, E.; Siwetz, M.; Guettler, J.; Huppertz, B. Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation. Int. J. Mol. Sci. 2021, 22, 7464. https://doi.org/10.3390/ijms22147464

AMA Style

Kupper N, Pritz E, Siwetz M, Guettler J, Huppertz B. Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation. International Journal of Molecular Sciences. 2021; 22(14):7464. https://doi.org/10.3390/ijms22147464

Chicago/Turabian Style

Kupper, Nadja, Elisabeth Pritz, Monika Siwetz, Jacqueline Guettler, and Berthold Huppertz. 2021. "Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation" International Journal of Molecular Sciences 22, no. 14: 7464. https://doi.org/10.3390/ijms22147464

APA Style

Kupper, N., Pritz, E., Siwetz, M., Guettler, J., & Huppertz, B. (2021). Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation. International Journal of Molecular Sciences, 22(14), 7464. https://doi.org/10.3390/ijms22147464

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