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Article

Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida

1
Department of Diagnostic and Interventional Radiology, University Hospital in Split, 21000 Split, Croatia
2
Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, 21000 Split, Croatia
3
Department of Biology, Faculty of Science, University of Split, 21000 Split, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: Pavla Jendelova
Int. J. Mol. Sci. 2021, 22(14), 7320; https://doi.org/10.3390/ijms22147320
Received: 29 April 2021 / Revised: 29 June 2021 / Accepted: 2 July 2021 / Published: 7 July 2021
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
The expression of 5-HT (serotonin) receptors (sr) was analyzed in the spinal cord and ganglia of 15 human conceptuses (5–10-weeks), and in the 9-week fetus with spina bifida. We used immunohistochemical method to detect sr-positive, apoptotic (caspase-3) and proliferating (Ki-67) cells, double immunofluorescence for co-localization with protein gene peptide (pgp) 9.5 and GFAP, as well as semiquantification and statistical measurements. Following the neurulation process, moderate (sr1 and sr2) and mild (sr3) expression characterized neuroblasts in the spinal cord and ganglia. During further development, sr1 expression gradually increased in the motoneurons, autonomic and sensory neurons, while sr2 and sr3 increased strongly in floor and roof plates. In the ganglia, sr3 expression increased during limited developmental period, while sr1 and sr2 increased throughout the investigated period. Co-expression of sr/pgp 9.5 characterized developing neurons, while sr/GFAP co-localized in the roof plate. In the spinal cord and ganglia of malformed fetus, weaker sr1 and sr2 and stronger sr3 expression accompanied morphological abnormalities. Anomalous roof plate morphology showed an excess of apoptotic and proliferating cells and increased sr3 expression. Our results indicate a human-species specific sr expression pattern, and the importance of sr1 in neuronal differentiation, and sr2 and sr3 in the control of the roof plate morphogenesis in normal and disturbed development. View Full-Text
Keywords: spinal cord; ganglia; human development; cervical spina bifida; serotonin spinal cord; ganglia; human development; cervical spina bifida; serotonin
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MDPI and ACS Style

Punda, H.; Mardesic, S.; Filipovic, N.; Kosovic, I.; Benzon, B.; Ogorevc, M.; Bocina, I.; Kolic, K.; Vukojevic, K.; Saraga-Babic, M. Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida. Int. J. Mol. Sci. 2021, 22, 7320. https://doi.org/10.3390/ijms22147320

AMA Style

Punda H, Mardesic S, Filipovic N, Kosovic I, Benzon B, Ogorevc M, Bocina I, Kolic K, Vukojevic K, Saraga-Babic M. Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida. International Journal of Molecular Sciences. 2021; 22(14):7320. https://doi.org/10.3390/ijms22147320

Chicago/Turabian Style

Punda, Hrvoje, Snjezana Mardesic, Natalija Filipovic, Ivona Kosovic, Benjamin Benzon, Marin Ogorevc, Ivana Bocina, Kresimir Kolic, Katarina Vukojevic, and Mirna Saraga-Babic. 2021. "Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida" International Journal of Molecular Sciences 22, no. 14: 7320. https://doi.org/10.3390/ijms22147320

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