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Article

Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy

1
Faculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, Norway
2
Faculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 61200 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Sébastien Bonhommeau
Int. J. Mol. Sci. 2021, 22(13), 6710; https://doi.org/10.3390/ijms22136710
Received: 19 May 2021 / Revised: 16 June 2021 / Accepted: 17 June 2021 / Published: 23 June 2021
Oleaginous filamentous fungi can accumulate large amount of cellular lipids and biopolymers and pigments and potentially serve as a major source of biochemicals for food, feed, chemical, pharmaceutical, and transport industries. We assessed suitability of Fourier transform (FT) Raman spectroscopy for screening and process monitoring of filamentous fungi in biotechnology. Six Mucoromycota strains were cultivated in microbioreactors under six growth conditions (three phosphate concentrations in the presence and absence of calcium). FT-Raman and FT-infrared (FTIR) spectroscopic data was assessed in respect to reference analyses of lipids, phosphorus, and carotenoids by using principal component analysis (PCA), multiblock or consensus PCA, partial least square regression (PLSR), and analysis of spectral variation due to different design factors by an ANOVA model. All main chemical biomass constituents were detected by FT-Raman spectroscopy, including lipids, proteins, cell wall carbohydrates, and polyphosphates, and carotenoids. FT-Raman spectra clearly show the effect of growth conditions on fungal biomass. PLSR models with high coefficients of determination (0.83–0.94) and low error (approximately 8%) for quantitative determination of total lipids, phosphates, and carotenoids were established. FT-Raman spectroscopy showed great potential for chemical analysis of biomass of oleaginous filamentous fungi. The study demonstrates that FT-Raman and FTIR spectroscopies provide complementary information on main fungal biomass constituents. View Full-Text
Keywords: oleaginous microorganisms; biodiesel; pigments; biopolymers; carotenoids; fatty acids; chitin; chitosan; fermentation; fungi oleaginous microorganisms; biodiesel; pigments; biopolymers; carotenoids; fatty acids; chitin; chitosan; fermentation; fungi
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MDPI and ACS Style

Dzurendová, S.; Shapaval, V.; Tafintseva, V.; Kohler, A.; Byrtusová, D.; Szotkowski, M.; Márová, I.; Zimmermann, B. Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy. Int. J. Mol. Sci. 2021, 22, 6710. https://doi.org/10.3390/ijms22136710

AMA Style

Dzurendová S, Shapaval V, Tafintseva V, Kohler A, Byrtusová D, Szotkowski M, Márová I, Zimmermann B. Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy. International Journal of Molecular Sciences. 2021; 22(13):6710. https://doi.org/10.3390/ijms22136710

Chicago/Turabian Style

Dzurendová, Simona, Volha Shapaval, Valeria Tafintseva, Achim Kohler, Dana Byrtusová, Martin Szotkowski, Ivana Márová, and Boris Zimmermann. 2021. "Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy" International Journal of Molecular Sciences 22, no. 13: 6710. https://doi.org/10.3390/ijms22136710

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