Caffeic Acid Phenethyl Ester-Incorporated Radio-Sensitive Nanoparticles of Phenylboronic Acid Pinacol Ester-Conjugated Hyaluronic Acid for Application in Radioprotection
Abstract
:1. Introduction
2. Results
2.1. Synthesis of HAsPBPE Conjugates
2.2. Preparation and Characterization of CAPE-Incorporated HAsPBPE Nanoparticles
2.3. Biological Activity of CAPE-Incorporated HAsPBPE Nanoparticles
2.4. In Vivo Animal Study
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Synthesis of HAsPBPE Conjugates
4.3. Characterization of Conjugates
4.4. Preparation of Nanoparticles
4.5. Characterization of Nanoparticles
4.6. Drug Release Study
4.7. Cell Culture
4.8. Fluorescence Observation of Nanoparticle Uptake into Cells
4.9. ROS Assay
4.10. In Vivo Radiation of Animal Model
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Polymer/Drug Weight Ratio (mg/mg) | Drug Contents (%, w/w) | Average Particle Size (nm) | |
---|---|---|---|
Theoretical | Experimental | ||
20/0 20/2 20/4 | - 9.1 16.7 | - 7.5 12.3 | 64.8 ± 4.8 82.1 ± 9.6 101.3 ± 12.3 |
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Choi, S.-H.; Lee, D.-Y.; Kang, S.; Lee, M.-K.; Lee, J.-H.; Lee, S.-H.; Lee, H.-L.; Lee, H.-Y.; Jeong, Y.-I. Caffeic Acid Phenethyl Ester-Incorporated Radio-Sensitive Nanoparticles of Phenylboronic Acid Pinacol Ester-Conjugated Hyaluronic Acid for Application in Radioprotection. Int. J. Mol. Sci. 2021, 22, 6347. https://doi.org/10.3390/ijms22126347
Choi S-H, Lee D-Y, Kang S, Lee M-K, Lee J-H, Lee S-H, Lee H-L, Lee H-Y, Jeong Y-I. Caffeic Acid Phenethyl Ester-Incorporated Radio-Sensitive Nanoparticles of Phenylboronic Acid Pinacol Ester-Conjugated Hyaluronic Acid for Application in Radioprotection. International Journal of Molecular Sciences. 2021; 22(12):6347. https://doi.org/10.3390/ijms22126347
Chicago/Turabian StyleChoi, Seon-Hee, Dong-Yeon Lee, Sohi Kang, Min-Kyung Lee, Jae-Heun Lee, Sang-Heon Lee, Hye-Lim Lee, Hyo-Young Lee, and Young-IL Jeong. 2021. "Caffeic Acid Phenethyl Ester-Incorporated Radio-Sensitive Nanoparticles of Phenylboronic Acid Pinacol Ester-Conjugated Hyaluronic Acid for Application in Radioprotection" International Journal of Molecular Sciences 22, no. 12: 6347. https://doi.org/10.3390/ijms22126347