Analysis of Both Lipid Metabolism and Endocannabinoid Signaling Reveals a New Role for Hypothalamic Astrocytes in Maternal Caloric Restriction-Induced Perinatal Programming
Abstract
:1. Introduction
2. Results
2.1. Effects of Caloric Restriction on Maternal Body Weight
2.2. Effects of Maternal Caloric Restriction on Markers of Gliosis and Neuroinflammation in PND2 Offspring Hypothalamus
2.3. Effects of Maternal Caloric Restriction on Lipid Metabolism and Endocannabinoid Signaling in PND2 Offspring Hypothalamus
2.4. Effects of Maternal Caloric Restriction on Lipid Metabolism and Endocannabinoid Signaling in Hypothalamic Astrocytes
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Animal Model and Diets
4.3. Sample Collection
4.4. Primary Cultures of Astrocytes
4.5. RNA Isolation and Real-Time Quantitative PCR Analysis
4.6. Western Blot Analysis
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Symbol | Assay ID | GenBank Accession Number | Amplicon Length (bp) |
---|---|---|---|
B2M | Rn00560865_m1 | NM_012512.2 | 58 |
Actb | Rn00667869_m1 | NM_031144.3 | 91 |
Srebf1 | Rn01495769_m1 | NM_001276707.1 | 79 |
Srebf2 | Rn01502638_m1 | NM_001033694.1 | 61 |
Cpt1c | Rn01475546_m1 | NM_001034925.2 | 88 |
Cpt1a | Rn00580702_m1 | NM_031559.2 | 64 |
Insr | Rn00573576_m1 | NM_022212.2 | 65 |
Acox1 | Rn01460628_m1 | NM_017340.2 | 63 |
Acaca | Rn00573474_m1 | NM_022193.1 | 60 |
Scd1 | Rn00594894_g1 | NM_139192.2 | 86 |
LepR | Rn01433205_m1 | NM_012596.1 | 94 |
Fasn | Rn01463550_m1 | NM_017332.1 | 148 |
Cnr1 | Rn02758689_s1 | NM_012784.4 | 92 |
Cnr2 | Rn01637601_m1 | NM_020543.4 | 68 |
Dagla | Rn01454303_m1 | NM_001005886.1 | 61 |
Daglb | Rn01453770_m1 | NM_001107120.1 | 57 |
Mgll | Rn00593297_m1 | NM_138502.2 | 78 |
Napepld | Rn01786262_m1 | NM_199381.1 | 71 |
Faah | Rn00577086_m1 | NM_024132.3 | 63 |
Vimentin | Rn00667825_m1 | NM_031140.1 | 83 |
Gfap | Rn01253033_m1 | NM_017009.2 | 75 |
Aif1 | Rn03993468_g1 | NM_017196.3 | 82 |
Antigen | Manufacturing | Dilution |
---|---|---|
ACC | Cell signal (C83B10) Rabbit mAb | 1/1000 |
ACOX1 | Abcam [EPR19038] ab184032 | 1/500 |
γ-adaptina | BD Biosciences. Mouse Adaptin 610385 | 1/2000 |
CPT1a | Abccam (ab102679) rabbit | 1/1000 |
FAS | Cell Signaling (C18C12) Rabbit mAb | 1/1000 |
SCD1 | Abcam [CD.E10] ab19862 | 1/250 |
Daglα | Biorbyt #orb-158553 | 1/100 |
Daglβ | Biorbyt #orb-182975 | 1/100 |
CB1 | Abcam #Ab23703 | 1/200 |
CB2 | Abcam #Ab3561 | 1/200 |
NAPEPLD | Abcam #Ab95397 | 1/1000 |
FAAH | CAYMAN #101600 | 1/100 |
GFAP | Thermofisher #MA5-12023 | 1/100 |
VIMENTINE | Thermofisher #MA5-11883 | 1/100 |
SBREP1 | Santa Cruz #sc-365513 | 1/100 |
SBREP2 | Santa Cruz #sc-13552 | 1/100 |
IBA1 | Wako | 1/500 |
MGLL | Abcam #Ab24701 | 1/200 |
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Tovar, R.; Vargas, A.; Aranda, J.; Sánchez-Salido, L.; González-González, L.; Chowen, J.A.; Rodríguez de Fonseca, F.; Suárez, J.; Rivera, P. Analysis of Both Lipid Metabolism and Endocannabinoid Signaling Reveals a New Role for Hypothalamic Astrocytes in Maternal Caloric Restriction-Induced Perinatal Programming. Int. J. Mol. Sci. 2021, 22, 6292. https://doi.org/10.3390/ijms22126292
Tovar R, Vargas A, Aranda J, Sánchez-Salido L, González-González L, Chowen JA, Rodríguez de Fonseca F, Suárez J, Rivera P. Analysis of Both Lipid Metabolism and Endocannabinoid Signaling Reveals a New Role for Hypothalamic Astrocytes in Maternal Caloric Restriction-Induced Perinatal Programming. International Journal of Molecular Sciences. 2021; 22(12):6292. https://doi.org/10.3390/ijms22126292
Chicago/Turabian StyleTovar, Rubén, Antonio Vargas, Jesús Aranda, Lourdes Sánchez-Salido, Laura González-González, Julie A. Chowen, Fernando Rodríguez de Fonseca, Juan Suárez, and Patricia Rivera. 2021. "Analysis of Both Lipid Metabolism and Endocannabinoid Signaling Reveals a New Role for Hypothalamic Astrocytes in Maternal Caloric Restriction-Induced Perinatal Programming" International Journal of Molecular Sciences 22, no. 12: 6292. https://doi.org/10.3390/ijms22126292