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Volume 22
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Article

Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing

by
Beata Kaczmarek-Szczepańska
1,
Justyna Ostrowska
1,
Justyna Kozłowska
1,
Zofia Szota
2,
Anna A. Brożyna
2,
Rita Dreier
3,
Russel J. Reiter
4,
Andrzej T. Slominski
5,6,
Kerstin Steinbrink
7 and
Konrad Kleszczyński
7,*
1
Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland
2
Department of Human Biology, Institute of Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland
3
Institute of Physiological Chemistry and Pathobiochemistry, Waldeyerstraße 15, 48149 Münster, Germany
4
Department of Cellular and Structural Biology, UT Health Science Center, San Antonio, TX 78229, USA
5
Comprehensive Cancer Center, Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
6
Pathology and Laboratory Medicine Service, VA Medical Center, Birmingham, AL 35294, USA
7
Department of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(11), 5658; https://doi.org/10.3390/ijms22115658
Submission received: 29 April 2021 / Revised: 17 May 2021 / Accepted: 25 May 2021 / Published: 26 May 2021
(This article belongs to the Special Issue Melatonin and Vitamin D: Molecular Mechanisms and Biological Function)
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Abstract

The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by (i) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (ii) thermogravimetric analysis (TG), (iii) scanning electron microscope (SEM), and (iv) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine.
Keywords: melatonin; scaffolds; biopolymers; collagen; chitosan; cutaneous cells; wound healing melatonin; scaffolds; biopolymers; collagen; chitosan; cutaneous cells; wound healing

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MDPI and ACS Style

Kaczmarek-Szczepańska, B.; Ostrowska, J.; Kozłowska, J.; Szota, Z.; Brożyna, A.A.; Dreier, R.; Reiter, R.J.; Slominski, A.T.; Steinbrink, K.; Kleszczyński, K. Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing. Int. J. Mol. Sci. 2021, 22, 5658. https://doi.org/10.3390/ijms22115658

AMA Style

Kaczmarek-Szczepańska B, Ostrowska J, Kozłowska J, Szota Z, Brożyna AA, Dreier R, Reiter RJ, Slominski AT, Steinbrink K, Kleszczyński K. Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing. International Journal of Molecular Sciences. 2021; 22(11):5658. https://doi.org/10.3390/ijms22115658

Chicago/Turabian Style

Kaczmarek-Szczepańska, Beata, Justyna Ostrowska, Justyna Kozłowska, Zofia Szota, Anna A. Brożyna, Rita Dreier, Russel J. Reiter, Andrzej T. Slominski, Kerstin Steinbrink, and Konrad Kleszczyński. 2021. "Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing" International Journal of Molecular Sciences 22, no. 11: 5658. https://doi.org/10.3390/ijms22115658

APA Style

Kaczmarek-Szczepańska, B., Ostrowska, J., Kozłowska, J., Szota, Z., Brożyna, A. A., Dreier, R., Reiter, R. J., Slominski, A. T., Steinbrink, K., & Kleszczyński, K. (2021). Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing. International Journal of Molecular Sciences, 22(11), 5658. https://doi.org/10.3390/ijms22115658

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