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Volume 22
Issue 11
10.3390/ijms22115638
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Article

Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation

by
Alexandra Calle
1,
Víctor Toribio
2,
María Yáñez-Mó
2,3 and
Miguel Ángel Ramírez
1,*
1
Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain
2
CBM-SO, Instituto de Investigación Sanitaria Princesa (IIS-IP), 28049 Madrid, Spain
3
Departamento de Biología Molecular, UAM, 28049 Madrid, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(11), 5638; https://doi.org/10.3390/ijms22115638
Submission received: 23 April 2021 / Revised: 19 May 2021 / Accepted: 21 May 2021 / Published: 26 May 2021
(This article belongs to the Special Issue Extracellular Vesicles in Reproduction—2nd Edition)
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Abstract

Embryonic implantation is a key step in the establishment of pregnancy. In the present work, we have carried out an in-depth proteomic analysis of the secretome (extracellular vesicles and soluble proteins) of two bovine blastocysts embryonic trophectoderm primary cultures (BBT), confirming different epithelial–mesenchymal transition stages in these cells. BBT-secretomes contain early pregnancy-related proteins and angiogenic proteins both as cargo in EVs and the soluble fraction. We have demonstrated the functional transfer of protein-containing secretome between embryonic trophectoderm and maternal MSC in vitro using two BBT primary cultures eight endometrial MSC (eMSC) and five peripheral blood MSC (pbMSC) lines. We observed that eMSC and pbMSC chemotax to both the soluble fraction and EVs of the BBT secretome. In addition, in a complementary direction, we found that the pattern of expression of implantation proteins in BBT-EVs changes depending on: (i) their epithelial–mesenchymal phenotype; (ii) as a result of the uptake of eMSC- or pbMSC-EV previously stimulated or not with embryonic signals (IFN-); (iii) because of the stimulation with the endometrial cytokines present in the uterine fluid in the peri-implantation period.
Keywords: mesenchymal stromal cells; trophectoderm; epithelial to mesenchymal transition; cell migration; extracellular vesicles mesenchymal stromal cells; trophectoderm; epithelial to mesenchymal transition; cell migration; extracellular vesicles

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MDPI and ACS Style

Calle, A.; Toribio, V.; Yáñez-Mó, M.; Ramírez, M.Á. Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation. Int. J. Mol. Sci. 2021, 22, 5638. https://doi.org/10.3390/ijms22115638

AMA Style

Calle A, Toribio V, Yáñez-Mó M, Ramírez MÁ. Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation. International Journal of Molecular Sciences. 2021; 22(11):5638. https://doi.org/10.3390/ijms22115638

Chicago/Turabian Style

Calle, Alexandra, Víctor Toribio, María Yáñez-Mó, and Miguel Ángel Ramírez. 2021. "Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation" International Journal of Molecular Sciences 22, no. 11: 5638. https://doi.org/10.3390/ijms22115638

APA Style

Calle, A., Toribio, V., Yáñez-Mó, M., & Ramírez, M. Á. (2021). Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation. International Journal of Molecular Sciences, 22(11), 5638. https://doi.org/10.3390/ijms22115638

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