Next Article in Journal
Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine Endometrium
Next Article in Special Issue
Effects of Aqueous Dispersions of C60, C70 and [email protected]82 Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways
Previous Article in Journal
Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach
Article

Ablation of Selenbp1 Alters Lipid Metabolism via the Pparα Pathway in Mouse Kidney

1
Laboratory of Molecular Life Sciences, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
2
Division of Pharmaceutical Cell Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
3
Laboratory of Hygienic Chemistry, Daiichi University of Pharmacy, 22-1 Tamagawa-cho, Minami-ku, Fukuoka 815-8511, Japan
4
Faculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Daigakudori 1-1-1, Sanyo-Onoda 756-0884, Japan
5
School of Pharmacy, International University of Health and Welfare Fukuoka, Ohkawa, Fukuoka 831-8501, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Maria Giulia Lionetto
Int. J. Mol. Sci. 2021, 22(10), 5334; https://doi.org/10.3390/ijms22105334
Received: 2 April 2021 / Revised: 10 May 2021 / Accepted: 15 May 2021 / Published: 19 May 2021
(This article belongs to the Special Issue Oxidative Stress and Genotoxicity: New Insights)
Selenium-binding protein 1 (Selenbp1) is a 2,3,7,8-tetrechlorodibenzo-p-dioxin inducible protein whose function is yet to be comprehensively elucidated. As the highly homologous isoform, Selenbp2, is expressed at low levels in the kidney, it is worthwhile comparing wild-type C57BL mice and Selenbp1-deficient mice under dioxin-free conditions. Accordingly, we conducted a mouse metabolomics analysis under non-dioxin-treated conditions. DNA microarray analysis was performed based on observed changes in lipid metabolism-related factors. The results showed fluctuations in the expression of numerous genes. Real-time RT-PCR confirmed the decreased expression levels of the cytochrome P450 4a (Cyp4a) subfamily, known to be involved in fatty acid ω- and ω-1 hydroxylation. Furthermore, peroxisome proliferator-activated receptor-α (Pparα) and retinoid-X-receptor-α (Rxrα), which form a heterodimer with Pparα to promote gene expression, were simultaneously reduced. This indicated that reduced Cyp4a expression was mediated via decreased Pparα and Rxrα. In line with this finding, increased levels of leukotrienes and prostaglandins were detected. Conversely, decreased hydrogen peroxide levels and reduced superoxide dismutase (SOD) activity supported the suppression of the renal expression of Sod1 and Sod2 in Selenbp1-deficient mice. Therefore, we infer that ablation of Selenbp1 elicits oxidative stress caused by increased levels of superoxide anions, which alters lipid metabolism via the Pparα pathway. View Full-Text
Keywords: selenium binding protein 1; peroxisome proliferator-activated receptor-alpha; Ppar; mouse; kidney; lipid metabolism; oxidative stress selenium binding protein 1; peroxisome proliferator-activated receptor-alpha; Ppar; mouse; kidney; lipid metabolism; oxidative stress
Show Figures

Graphical abstract

MDPI and ACS Style

Song, Y.; Kurose, A.; Li, R.; Takeda, T.; Onomura, Y.; Koga, T.; Mutoh, J.; Ishida, T.; Tanaka, Y.; Ishii, Y. Ablation of Selenbp1 Alters Lipid Metabolism via the Pparα Pathway in Mouse Kidney. Int. J. Mol. Sci. 2021, 22, 5334. https://doi.org/10.3390/ijms22105334

AMA Style

Song Y, Kurose A, Li R, Takeda T, Onomura Y, Koga T, Mutoh J, Ishida T, Tanaka Y, Ishii Y. Ablation of Selenbp1 Alters Lipid Metabolism via the Pparα Pathway in Mouse Kidney. International Journal of Molecular Sciences. 2021; 22(10):5334. https://doi.org/10.3390/ijms22105334

Chicago/Turabian Style

Song, Yingxia, Atsushi Kurose, Renshi Li, Tomoki Takeda, Yuko Onomura, Takayuki Koga, Junpei Mutoh, Takumi Ishida, Yoshitaka Tanaka, and Yuji Ishii. 2021. "Ablation of Selenbp1 Alters Lipid Metabolism via the Pparα Pathway in Mouse Kidney" International Journal of Molecular Sciences 22, no. 10: 5334. https://doi.org/10.3390/ijms22105334

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop