Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures

Sacchetto, Claudia; Vitiello, Libero; de Windt, Leon J.; Rampazzo, Alessandra; Calore, Martina

doi:10.3390/ijms21093404

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Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures

^1,2,†,

^2,3,†

^2,*

and

Martina Calore

^1,*

Department of Molecular Genetics, University of Maastricht, Universiteitssingel 50, 6229ER Maastricht, The Netherlands

Department of Biology, University of Padova, via Ugo Bassi 58B, 35131 Padova, Italy

Interuniversity Institute of Myology (IIM), Administrative headquarters University of Perugia, Piazza Lucio Severi 1, 06132 Perugia, Italy

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2020, 21(9), 3404; https://doi.org/10.3390/ijms21093404

Received: 21 April 2020 / Revised: 5 May 2020 / Accepted: 6 May 2020 / Published: 11 May 2020

(This article belongs to the Special Issue Disease Modeling Using Human Induced Pluripotent Stem Cells 2.0)

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Abstract

In the last decade, the generation of cardiac disease models based on human-induced pluripotent stem cells (hiPSCs) has become of common use, providing new opportunities to overcome the lack of appropriate cardiac models. Although much progress has been made toward the generation of hiPSC-derived cardiomyocytes (hiPS-CMs), several lines of evidence indicate that two-dimensional (2D) cell culturing presents significant limitations, including hiPS-CMs immaturity and the absence of interaction between different cell types and the extracellular matrix. More recently, new advances in bioengineering and co-culture systems have allowed the generation of three-dimensional (3D) constructs based on hiPSC-derived cells. Within these systems, biochemical and physical stimuli influence the maturation of hiPS-CMs, which can show structural and functional properties more similar to those present in adult cardiomyocytes. In this review, we describe the latest advances in 2D- and 3D-hiPSC technology for cardiac disease mechanisms investigation, drug development, and therapeutic studies. View Full-Text

Keywords: cardiac disease modeling; human induced pluripotent stem cells; 3D cardiac models; engineered heart tissue

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Sacchetto, C.; Vitiello, L.; de Windt, L.J.; Rampazzo, A.; Calore, M. Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures. Int. J. Mol. Sci. 2020, 21, 3404. https://doi.org/10.3390/ijms21093404

AMA Style

Sacchetto C, Vitiello L, de Windt LJ, Rampazzo A, Calore M. Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures. International Journal of Molecular Sciences. 2020; 21(9):3404. https://doi.org/10.3390/ijms21093404

Chicago/Turabian Style

Sacchetto, Claudia, Libero Vitiello, Leon J. de Windt, Alessandra Rampazzo, and Martina Calore. 2020. "Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures" International Journal of Molecular Sciences 21, no. 9: 3404. https://doi.org/10.3390/ijms21093404

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Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures

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